Douglas Gjerde received his PhD in analytical chemistry from Iowa State University (USA). After having worked in large and small companies for a number of years, Dr. Gjerde founded Sarasep (now merged into Transgenomic) in 1990 for the production and commercialization of nucleic acid separation technologies. He has authored and coauthored numerous articles and 4 books and holds nearly fifty patents in separation science. Lee Hoang is a graduate of the University of California, Santa Cruz (USA) where he obtained a Ph.D. in Molecular Biology in 2006. He is now an applications scientist at PhyNexus in San Jose, a company specializing on protein purification that was founded by Douglas Gjerde. David Hornby studied biochemistry at the University of Sheffield (UK). Since 1999 he is a full professor for Molecular Biology and Biotechnology at the same institution. He has also co-founded two start-up companies (Invector and Protealysis), based on novel technologies for RNA separation and stabilisation, for which he holds the patents. His current research focuses on RNAi biochemistry and proteomics technologies.

• RNA EXTRACTION, SEPARATION AND ANALYSISThe need to be able to extract, manipulate and analyze RNAUsing chemical tools to solve the problem of analysis of biological problemsThe principle of chromatography and solid phase extractionRNA ChromatographyEnzymatic treatment of RNA and analysisContent and organization of this book BIOLOGICAL AND CHEMICAL RNA Why Classify RNA with biology and chemistry?Prokaryote cellular RNAProkaryote sample typeEukaryote cellular RNAEukaryote sample typeOther Samples (Virus, Soil, Rock)Synthetic RNA RNA SEPARATION -SUBSTRATES, FUNCTIONAL GROUPS, MECHANISMS AND CONTROLSolid phase interactionThe solid phase substrate and attachment of functional groupsReverse phase ion pairing separation mechanismIon exchange separation mechanismChaotropic denaturing interaction mechanism Hybridization /SELEXGel Filtration RNA EXTRACTION AND ANALYSISTranscriptionTranslationGene regulationUse of siRNA to investigate gene function RNA CHROMATOGRAPHY Development of RNA Chromatography RNA Chromatography instrumentationRNA Chromatography conditionsTemperature modes of RNA ChromatographyComparison of gel electrophoresis and liquid chromatographyAnalysis of human telomerase RNA under non denaturing conditions RNA CHROMATOGRAPHY, SEPARATION AND ANALYSISFeatures of RNA ChromatographySeparation of double-stranded and single-stranded RNASeparation of cellular RNA speciesSeparation of messenger RNA from total and ribosomal RNAAnalysis of transfer RNAChromatography and analysis of synthetic oligo ribonucleotidesApplication of RNA and DNA Chromatography in cDNA library synthesisChromatography analyses of RT-PCR and competitive RT-PCR productsAlternative splicingDifferential messenger RNA display via RNA Chromatography RNA STRUCTURE-FUNCTION PROBINGDefinition of the Structure-function paradigmStructure determination of RNAFootprinting, model building and functional investigations APPENDIXChromatographic Separation Equotations and Priciples for RNA SeparationHPLC Instrumentation and Operation RNA Chromatographic System Cleaning and Passivation Treatment

Douglas Gjerde received his PhD in analytical chemistry from Iowa State University (USA). After having worked in large and small companies for a number of years, Dr. Gjerde founded Sarasep (now merged into Transgenomic) in 1990 for the production and commercialization of nucleic acid separation technologies. He has authored and coauthored numerous articles and 4 books and holds nearly fifty patents in separation science. Lee Hoang is a graduate of the University of California, Santa Cruz (USA) where he obtained a Ph.D. in Molecular Biology in 2006. He is now an applications scientist at PhyNexus in San Jose, a company specializing on protein purification that was founded by Douglas Gjerde. David Hornby studied biochemistry at the University of Sheffield (UK). Since 1999 he is a full professor for Molecular Biology and Biotechnology at the same institution. He has also co-founded two start-up companies (Invector and Protealysis), based on novel technologies for RNA separation and stabilisation, for which he holds the patents. His current research focuses on RNAi biochemistry and proteomics technologies.

• RNA EXTRACTION, SEPARATION AND ANALYSISThe need to be able to extract, manipulate and analyze RNAUsing chemical tools to solve the problem of analysis of biological problemsThe principle of chromatography and solid phase extractionRNA ChromatographyEnzymatic treatment of RNA and analysisContent and organization of this book BIOLOGICAL AND CHEMICAL RNA Why Classify RNA with biology and chemistry?Prokaryote cellular RNAProkaryote sample typeEukaryote cellular RNAEukaryote sample typeOther Samples (Virus, Soil, Rock)Synthetic RNA RNA SEPARATION -SUBSTRATES, FUNCTIONAL GROUPS, MECHANISMS AND CONTROLSolid phase interactionThe solid phase substrate and attachment of functional groupsReverse phase ion pairing separation mechanismIon exchange separation mechanismChaotropic denaturing interaction mechanism Hybridization /SELEXGel Filtration RNA EXTRACTION AND ANALYSISTranscriptionTranslationGene regulationUse of siRNA to investigate gene function RNA CHROMATOGRAPHY Development of RNA Chromatography RNA Chromatography instrumentationRNA Chromatography conditionsTemperature modes of RNA ChromatographyComparison of gel electrophoresis and liquid chromatographyAnalysis of human telomerase RNA under non denaturing conditions RNA CHROMATOGRAPHY, SEPARATION AND ANALYSISFeatures of RNA ChromatographySeparation of double-stranded and single-stranded RNASeparation of cellular RNA speciesSeparation of messenger RNA from total and ribosomal RNAAnalysis of transfer RNAChromatography and analysis of synthetic oligo ribonucleotidesApplication of RNA and DNA Chromatography in cDNA library synthesisChromatography analyses of RT-PCR and competitive RT-PCR productsAlternative splicingDifferential messenger RNA display via RNA Chromatography RNA STRUCTURE-FUNCTION PROBINGDefinition of the Structure-function paradigmStructure determination of RNAFootprinting, model building and functional investigations APPENDIXChromatographic Separation Equotations and Priciples for RNA SeparationHPLC Instrumentation and Operation RNA Chromatographic System Cleaning and Passivation Treatment

Douglas Gjerde received his PhD in analytical chemistry from Iowa State University (USA). After having worked in large and small companies for a number of years, Dr. Gjerde founded Sarasep (now merged into Transgenomic) in 1990 for the production and commercialization of nucleic acid separation technologies. He has authored and coauthored numerous articles and 4 books and holds nearly fifty patents in separation science. Lee Hoang is a graduate of the University of California, Santa Cruz (USA) where he obtained a Ph.D. in Molecular Biology in 2006. He is now an applications scientist at PhyNexus in San Jose, a company specializing on protein purification that was founded by Douglas Gjerde. David Hornby studied biochemistry at the University of Sheffield (UK). Since 1999 he is a full professor for Molecular Biology and Biotechnology at the same institution. He has also co-founded two start-up companies (Invector and Protealysis), based on novel technologies for RNA separation and stabilisation, for which he holds the patents. His current research focuses on RNAi biochemistry and proteomics technologies.

• RNA EXTRACTION, SEPARATION AND ANALYSISThe need to be able to extract, manipulate and analyze RNAUsing chemical tools to solve the problem of analysis of biological problemsThe principle of chromatography and solid phase extractionRNA ChromatographyEnzymatic treatment of RNA and analysisContent and organization of this book BIOLOGICAL AND CHEMICAL RNA Why Classify RNA with biology and chemistry?Prokaryote cellular RNAProkaryote sample typeEukaryote cellular RNAEukaryote sample typeOther Samples (Virus, Soil, Rock)Synthetic RNA RNA SEPARATION -SUBSTRATES, FUNCTIONAL GROUPS, MECHANISMS AND CONTROLSolid phase interactionThe solid phase substrate and attachment of functional groupsReverse phase ion pairing separation mechanismIon exchange separation mechanismChaotropic denaturing interaction mechanism Hybridization /SELEXGel Filtration RNA EXTRACTION AND ANALYSISTranscriptionTranslationGene regulationUse of siRNA to investigate gene function RNA CHROMATOGRAPHY Development of RNA Chromatography RNA Chromatography instrumentationRNA Chromatography conditionsTemperature modes of RNA ChromatographyComparison of gel electrophoresis and liquid chromatographyAnalysis of human telomerase RNA under non denaturing conditions RNA CHROMATOGRAPHY, SEPARATION AND ANALYSISFeatures of RNA ChromatographySeparation of double-stranded and single-stranded RNASeparation of cellular RNA speciesSeparation of messenger RNA from total and ribosomal RNAAnalysis of transfer RNAChromatography and analysis of synthetic oligo ribonucleotidesApplication of RNA and DNA Chromatography in cDNA library synthesisChromatography analyses of RT-PCR and competitive RT-PCR productsAlternative splicingDifferential messenger RNA display via RNA Chromatography RNA STRUCTURE-FUNCTION PROBINGDefinition of the Structure-function paradigmStructure determination of RNAFootprinting, model building and functional investigations APPENDIXChromatographic Separation Equotations and Priciples for RNA SeparationHPLC Instrumentation and Operation RNA Chromatographic System Cleaning and Passivation Treatment