Flow Cytometry and Cell Sorting

Häftad, Engelska, 2010

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Beskrivning

The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories.A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Produktinformation

Utgivningsdatum:2010-12-15
Mått:203 x 254 x 21 mm
Vikt:928 g
Format:Häftad
Språk:Engelska
Serie:Springer Lab Manuals
Antal sidor:355
Upplaga:2
Förlag:Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
ISBN:9783642084928

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Onkologi inom Medicin
Cellbiologi inom Naturvetenskap och teknik

Innehållsförteckning

I Flow Cytometry.- 1 Operation of a Flow Cytometer.- II Immunofluorescence.- 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies.- 3 Immunofluorescence: Basic Considerations.- 4 Multicolor Immunofluorescence Analysis.- 5 Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells.- 6 Cytometry and Sorting of Live Cells According to Their Secreted Products.- 7 Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes.- 8 Scatchard Analysis by Flow Cytometry.- 9 Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry.- III DNA and Proliferation.- 10 Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis.- 11 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression.- 12 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique.- IV Cellular Activation and Biochemistry.- 13 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium.- 14 Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry.- 15 Biochemical Parameters of Cells Function.- 16 The FACS-Gal Assay.- 17 Ligand Acidification by Nonadherent Cells.- V Cell Sorting.- 18 Powerful Preselection.- 19 Magnetic Selection of Transfected Cells.- 20 High Gradient Magnetic Cells Sorting.- 21 Setup of a Flow Sorter.- 22 Sorting of Rare Cells.- 23 Fluorescence-Activated Chromosome Sorting.- 24 Analysis and Sorting of Plant Chromosomes by Flow Cytometry.- 25 Large Particle Sorting.- VI Safety.- 26 Biological and Laser Safety.- Appendices: Birgit Mechtold and Andreas Radbruch.- Suppliers.- Cytometry on the Internet.