Springer Lab Manuals – serie
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59 produkter
Maize Handbook
Av Freeling, Michael Freeling m. fl.
Häftad, 1996
1643 kr
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The Maize Handbook represents the collective efforts of the maize research community to enumerate the key steps of standard procedures and to disseminate these protocols for the common good. Although the material in this volume is drawn from experience with maize, many of the procedures, protocols, …
Methods in Endothelial Cell Biology
Häftad, 2004
2253 kr
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Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most …
Basic Methods for the Biochemical Lab
Häftad, 2006
1425 kr
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Basic Methods for the Biochemical Lab elucidates proven lab procedures and practical hints for research in analytical and preparative biochemistry, as well as summarizing key data in numerous tables. To further emphasize the practical aspects and to minimize the text, theoretical introductions into …
Protocols in Lichenology
Culturing, Biochemistry, Ecophysiology and Use in Biomonitoring
Av Ilse Kranner, Richard Beckett m. fl.
Häftad, 2001
2733 kr
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As an intricate association between a fungus and one or more green algae or cyanobacteria, lichens are one of the most successful examples of symbiosis. These fascinating organisms survive extreme desiccation and temperatures. They are adapted to a great variety of habitats, from deserts to …
Peptide Arrays on Membrane Supports
Synthesis and Applications
Av Joachim Koch, Michael Mahler
Häftad, 2002
1643 kr
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Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding.The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding …
Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology
Practical Protocols
Av Marita E. Metz-Becker, K. Breunig m. fl.
Häftad, 2003
1643 kr
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Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the …
Principles of Peptide Synthesis
Häftad, 1993
1425 kr
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Principles of Peptide Synthesis is a very successful book by one of the pioneers of contemporary bioorganic synthesis. Its first edition proved a valuable source that is kept close to the work bench for frequent consulting. Any researcher planning the formation of the peptide bond, be it for the …
Practice of Peptide Synthesis
Av Miklos Bodanszky, Agnes Bodanszky
Häftad, 1994
1098 kr
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During the years 1980-81, as guests of the Deutsches Wollforschungsinstitut in Aachen, Germany, we were working on a small book entitled, "Principles of Peptide Synthesis". In the library of the Institute we noted that the volumes of Houben-Weyl's Handbuch der Organischen Chemie dealing with …
Cell Cycle - Materials and Methods
Häftad, 1995
2188 kr
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During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New …
Nucleic Acids in the Environment
Av Jack T. Trevors, J.Dick van Elsas
Häftad, 1995
1098 kr
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Molecular biological techniques such as DNA/RNA extraction and purification, and especially the polymerase chain reaction, PCR, are rapidly gaining interest also in related fields, such as microbiology or environmental sciences. They offer new approaches and opportunities for the determination of …
Microinjection and Transgenesis
Strategies and Protocols
Av Angel Cid-Arregui, Alejandro Garcia-Carranca
Häftad, 1997
569 kr
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The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches …
Animal Cell Culture Techniques
Häftad, 1998
1534 kr
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Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are …
Differential-Display Reverse Transcription-PCR (DDRT-PCR)
Av Sergio Colonna-Romano, Antonella Leone m. fl.
Häftad, 1998
1098 kr
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Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.Recently, a new …
Nonradioactive Analysis of Biomolecules
Av Christoph Kessler, Christoph Kessler
Häftad, 2000
1098 kr
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New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical …
Flow Cytometry and Cell Sorting
Häftad, 1999
2188 kr
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Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these …
Basic Techniques in Molecular Biology
Häftad, 2000
3606 kr
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This laboratory manual gives a thorough introduction to basic techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. …
Electrotransformation of Bacteria
Av Natalie Eynard, Justin Teissie
Häftad, 2000
1643 kr
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Creating bacteria with modified genetic properties allows the specific investigation of these microorganisms. Electrotransformation is a highly efficient and easy to apply technique to introduce genetic material into bacterial cells. A strong electric field is used for this purpose. In the present …
Resin Microscopy and On-Section Immunocytochemistry
Av Geoffrey R. Newman, Jan A. Hobot
Inbunden, 2001
2253 kr
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Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly …
Peptide Arrays on Membrane Supports
Synthesis and Applications
Av Joachim Koch, Michael Mahler
Häftad, 2010
2316 kr
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Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding.The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding …
Flow Cytometry and Cell Sorting
Häftad, 2010
2946 kr
Lägg i varukorg
Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these …
Electrotransformation of Bacteria
Av Natalie Eynard, Justin Teissie
Häftad, 2010
2421 kr
Lägg i varukorg
Creating bacteria with modified genetic properties allows the specific investigation of these microorganisms. Electrotransformation is a highly efficient and easy to apply technique to introduce genetic material into bacterial cells. A strong electric field is used for this purpose. In the present …
Resin Microscopy and On-Section Immunocytochemistry
Av Geoffrey R. Newman, Jan A. Hobot
Häftad, 2014
1692 kr
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Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly …
Genetic Library Construction and Screening
Advanced Techniques and Applications
Häftad, 2012
551 kr
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Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging …
Measuring Calcium and Calmodulin Inside and Outside Cells
Häftad, 2014
1128 kr
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The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signaling molecules as well as many pathophysiological processes, including …
FISH Technology
Av Bernd W. Rautenstrauß, Thomas Liehr
Häftad, 2012
1128 kr
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Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and …
Nucleic Acid Electrophoresis
Häftad, 2012
551 kr
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Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, …
Protein Structure Analysis
Preparation, Characterization, and Microsequencing
Av Roza Maria Kamp, Theodora Choli-Papadopoulou m. fl.
Häftad, 2012
551 kr
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"Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide …
Proteolytic Enzymes
Tools and Targets
Av Erwin E. Sterchi, Walter Stöcker
Häftad, 2014
1098 kr
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Researchers may find themselves confronted with proteases, either because they play an essential role in a particular process they are studying, or because they interfere with that process. In either case they may need to investigate or inhibit the proteolytic activity. Others may wish to use …
Techniques in Molecular Medicine
Av Friedhelm Hildebrandt, Peter Igarashi
Häftad, 2014
1128 kr
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Standard molecular biology techniques are used widely in biomedical re search and have had a major impact on the understanding of physiologic processes and disease mechanisms. They also play an increasing role in mo lecular genetic diagnosis and new therapeutic approaches. This laboratory manual …
Analytical Parasitology
Häftad, 2013
1128 kr
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Parasitic diseases still affect millions of people every year, especially in the tropics, causing considerable morbidity or death. Such infections within livestock are probably an even bigger problem, leading to poorer productivity, condemna tion of infected meat and considerable economic loss. …
