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7 produkter

Practical Guide to Protein and Peptide Purification for Microsequencing
AvPaul T. Matsudaira
E-bok
PDF, Engelska, 2012
695 kr
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Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.- Mass spectrophotometric characterization of proteins and peptides- N-terminal sequencing of proteins with blocked N termini- Internal amino acid sequence analysis after protease digestion in-gel and on-blot- Improved microscale peptide purification methods- Computer analysis of protein sequences- New protocols tested and refined through everyday use in authors'' laboratories- Updated reference chapter covering all aspects of protein microsequencing
Del 73 - Methods in Cell Biology
Cumulative Index, Volumes
AvLeslie Wilson,Paul T. Matsudaira
Inbunden, Engelska, 2003
1 827 kr
Skickas inom 10-15 vardagar
This is a cumulative index of Volumes 53-71 of the Methods in Cell Biology series. Critically acclaimed for more than 25 years, the series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users.
A Practical Guide to Protein and Peptide Purification for Microsequencing
AvPaul T. Matsudaira,Paul T. Matsudaira
Häftad, Engelska, 1993
555 kr
Skickas inom 10-15 vardagar
Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing
Del 40 - Methods in Cell Biology
A Practical Guide to the Study of Calcium in Living Cells
AvLeslie Wilson,Paul T. Matsudaira
Häftad, Engelska, 1994
701 kr
Skickas inom 10-15 vardagar
A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes Fluorescent dyes Luminescent proteins Includes techniques for perturbing intracellular Ca2+ Covers detailed methodology plus problems and pitfalls of each technique Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program Color plates illustrate techniques such as Confocal ratio-imaging Use of aequorin
Del 68 - Methods in Cell Biology
Atomic Force Microscopy in Cell Biology
AvLeslie Wilson,Paul T. Matsudaira
Inbunden, Engelska, 2002
2 096 kr
Skickas inom 10-15 vardagar
This is the first book to cover the history, structure, and application of atomic force microscopy in cell biology. Presented in the clear, well-illustrated style of the Methods in Cell Biology series, it introduces the AFM to its readers and enables them to tap the power and scope of this technology to further their own research. A practical laboratory guide for use of the atomic force and photonic force microscopes, it provides updated technology and methods in force spectroscopy. It is also a comprehensive and easy-to-follow practical laboratory guide for the use of the AFM and PFM in biological research.
Del 40 - Methods in Cell Biology
A Practical Guide to the Study of Calcium in Living Cells
AvLeslie Wilson,Paul T. Matsudaira
Inbunden, Engelska, 1994
701 kr
Skickas inom 10-15 vardagar
A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes Fluorescent dyes Luminescent proteins Includes techniques for perturbing intracellular Ca2+ Covers detailed methodology plus problems and pitfalls of each technique Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program Color plates illustrate techniques such as Confocal ratio-imaging Use of aequorin
Del 56 - Methods in Cell Biology
Video Microscopy
AvLeslie Wilson,Paul T. Matsudaira
Häftad, Engelska, 1998
1 312 kr
Skickas inom 10-15 vardagar
Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques. Chapters cover the commonly used video technologies in biological research Nontechnical presentation of principles Emphasis on the practical aspects of instrument use Covers pitfalls in instrument uses that can lead to artifacts Authors are leaders in the design and application of video methods to biological microscopy Presentation of material tailored for the established researcher that has little experience with video methods Examples are extensively illustrated with photographs

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Paul T. Matsudaira – författare

Practical Guide to Protein and Peptide Purification for Microsequencing

Cumulative Index, Volumes

A Practical Guide to Protein and Peptide Purification for Microsequencing

A Practical Guide to the Study of Calcium in Living Cells

Atomic Force Microscopy in Cell Biology

A Practical Guide to the Study of Calcium in Living Cells

Video Microscopy

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