Concepts and Strategies in Plant Sciences – serie

This book provides in-situ phytoremediation strategies that are particularly well suited for developing nations. Its goal is to promote the use of field-tested phytoremediation methods for removing soil and water pollutants from agricultural, industrial, military, and municipal sources. These strategies include using algae and a variety of aquatic and terrestrial plants. The book subsequently discusses the use of crops and native plants for phytoremediation, and how phytoremediation efforts impact the rhizosphere. After having finished the book, readers will be able to directly adapt the strategies described here for their specific purposes.

This book summarizes the latest findings on the functions of microRNAs in the regulation of plant development and responses to the surrounding environment.

This book summarizes the latest findings on the functions of microRNAs in the regulation of plant development and responses to the surrounding environment. MicroRNAs are an important class of molecules that can be found in diverse groups of organisms, including plants and animals, and the investigation of their roles is a highly dynamic and “hot” research topic. The respective chapters address four main aspects, namely: microRNA investigation and annotation, the regulatory roles of microRNAs in various developmental processes, in response to abiotic factors, and in the context of biotic stress response regulation. Systematically reviewing the most important findings in this field, the book offers an essential guide for undergraduate and graduate students, teachers, and plant science researchers. Due to the potential applications of microRNAs in crop breeding and plant protection, it also represents a valuable resource for scientists in academia and the private sector alike.

This book offers a comprehensive collection of papers on CRISPR/Cas genome editing in connection with agriculture, climate-smart crops, food security, translational research applications, bioinformatics analysis, practical applications in cereals, floriculture crops, engineering plants for abiotic stress resistance, the intellectual landscape, regulatory framework, and policy decisions. Gathering contributions by internationally respected experts in the field of CRISPR/Cas genome editing, the book offers an essential guide for researchers, students, teachers and scientists in academia; policymakers; and public companies, private companies and cooperatives interested in understanding and/or applying CRISPR/Cas genome editing to develop new agricultural products.

This book offers a comprehensive collection of papers on CRISPR/Cas genome editing in connection with agriculture, climate-smart crops, food security, translational research applications, bioinformatics analysis, practical applications in cereals, floriculture crops, engineering plants for abiotic stress resistance, the intellectual landscape, regulatory framework, and policy decisions. Gathering contributions by internationally respected experts in the field of CRISPR/Cas genome editing, the book offers an essential guide for researchers, students, teachers and scientists in academia; policymakers; and public companies, private companies and cooperatives interested in understanding and/or applying CRISPR/Cas genome editing to develop new agricultural products.

This book serves the teachers, researchers and the students as a handy and concise reference as well as guidebook while designing and planning for use of the advanced technologies for crop improvement.The content of the book is designed to cover the latest genome engineering techniques for crop improvement. The conventional breeding has got its limitations such as non-availability of desired genes within the genepool. In many cases, breeding has been highly used and it has nearly reached its highest limit so far as the productivity and production of crops are concerned. However, with increasing need of food and decreasing resources, including water, land, labour, etc., to feed the growing population, the alternative available ways of increasing crop productivity need to be explored and exploited. Genome engineering has a wide scope that includes technologies such as genetic engineering and transgenesis, RNA technologies, CRISPR, cisgenics and subgenics for better productivityand more efficient biotic and abiotic stress management. Therefore, the book is planned to enlighten the readers with the advanced technologies with examples and case studies, whenever possible. Efforts will be made to emphasize on general efforts on various major food crops; however, it would also be made clear that such efforts could be taken as proofs of concepts and that this could be extrapolated keeping the demand in mind.

This book serves the teachers, researchers and the students as a handy and concise reference as well as guidebook while designing and planning for use of the advanced technologies for crop improvement.The content of the book is designed to cover the latest genome engineering techniques for crop improvement. The conventional breeding has got its limitations such as non-availability of desired genes within the genepool. In many cases, breeding has been highly used and it has nearly reached its highest limit so far as the productivity and production of crops are concerned. However, with increasing need of food and decreasing resources, including water, land, labour, etc., to feed the growing population, the alternative available ways of increasing crop productivity need to be explored and exploited. Genome engineering has a wide scope that includes technologies such as genetic engineering and transgenesis, RNA technologies, CRISPR, cisgenics and subgenics for better productivityand more efficient biotic and abiotic stress management. Therefore, the book is planned to enlighten the readers with the advanced technologies with examples and case studies, whenever possible. Efforts will be made to emphasize on general efforts on various major food crops; however, it would also be made clear that such efforts could be taken as proofs of concepts and that this could be extrapolated keeping the demand in mind.

This book offers a unique and comprehensive overview of key RNA-based technologies, as well as their development and applications for the functional genomics of plant coding and non-coding genes. It focuses on the latest as well as classical RNA-based techniques used for studies on small RNAs, long non-coding RNAs and protein-coding genes. These techniques chiefly focus on target mimics (TMs) and short tandem target mimics (STTMs) for small RNAs, and artificial microRNAs (amiRNAs), RNA interference (RNAi)  and CRISPR/Cas for genes.   Furthermore, the book discusses the latest trends in the field and various modifications of the above-mentioned approaches, and explores how these RNA-based technologies have been developed, applied and validated as essential technologies in plant functional genomics. RNA-based technologies, their mechanisms of action, their advantages and disadvantages, and insights into the further developmentand applications of these technologies in plants are discussed. These techniques will enable the users to functionally characterize genes and small RNAs through silencing, overexpression and editing. Gathering contributions by globally respected experts, the book will appeal to students, teachers and scientists in academia and industry who are interested in horticulture, genetics, pathology, entomology, physiology, molecular genetics and breeding, in vitro culture & genetic engineering, and functional genomics.

This book offers a unique and comprehensive overview of key RNA-based technologies, as well as their development and applications for the functional genomics of plant coding and non-coding genes. It focuses on the latest as well as classical RNA-based techniques used for studies on small RNAs, long non-coding RNAs and protein-coding genes. These techniques chiefly focus on target mimics (TMs) and short tandem target mimics (STTMs) for small RNAs, and artificial microRNAs (amiRNAs), RNA interference (RNAi)  and CRISPR/Cas for genes.   Furthermore, the book discusses the latest trends in the field and various modifications of the above-mentioned approaches, and explores how these RNA-based technologies have been developed, applied and validated as essential technologies in plant functional genomics. RNA-based technologies, their mechanisms of action, their advantages and disadvantages, and insights into the further developmentand applications of these technologies in plants are discussed. These techniques will enable the users to functionally characterize genes and small RNAs through silencing, overexpression and editing. Gathering contributions by globally respected experts, the book will appeal to students, teachers and scientists in academia and industry who are interested in horticulture, genetics, pathology, entomology, physiology, molecular genetics and breeding, in vitro culture & genetic engineering, and functional genomics.

This book reviews the application of nanosensors in food and agriculture. Nanotechnology has the potential to become transformative technology that will impact almost all sectors. Tools like nanosensors, which detect specific molecular interactions, can be used for on-site, in-situ and online measurements of various parameters in clinical diagnostics, environmental and food monitoring, and quality control.Due to their unprecedented performance and sensitivity, nanobiosensors are gaining importance in precision farming. The book examines the use of nanobiosensors in the monitoring of food additives, toxins and mycotoxins, microbial contamination, food allergens, nutritional constituents, pesticides, environmental parameters, plant diseases and genetically modified organisms. It also discusses the role of biosensors in increasing crop productivity in sustainable agriculture, and nanosensor-based smart delivery systems to optimize the use of natural resources such as water, nutrients and agrochemicals in precision farming.

This book reviews the application of nanosensors in food and agriculture. Nanotechnology has the potential to become transformative technology that will impact almost all sectors. Tools like nanosensors, which detect specific molecular interactions, can be used for on-site, in-situ and online measurements of various parameters in clinical diagnostics, environmental and food monitoring, and quality control.Due to their unprecedented performance and sensitivity, nanobiosensors are gaining importance in precision farming. The book examines the use of nanobiosensors in the monitoring of food additives, toxins and mycotoxins, microbial contamination, food allergens, nutritional constituents, pesticides, environmental parameters, plant diseases and genetically modified organisms. It also discusses the role of biosensors in increasing crop productivity in sustainable agriculture, and nanosensor-based smart delivery systems to optimize the use of natural resources such as water, nutrients and agrochemicals in precision farming.

This book provides an overview of the innovations in crop phenotyping using emerging technologies, i.e., high-throughput crop phenotyping technology, including its concept, importance, breakthrough and applications in different crops and environments.

This book provides an overview of the innovations in crop phenotyping using emerging technologies, i.e., high-throughput crop phenotyping technology, including its concept, importance, breakthrough and applications in different crops and environments. Emerging technologies in sensing, machine vision and high-performance computing are changing the world beyond our imagination. They are also becoming the most powerful driver of the innovation in agriculture technology, including crop breeding, genetics and management. It includes the state of the art of technologies in high-throughput phenotyping, including advanced sensors, automation systems, ground-based or aerial robotic systems. It also discusses the emerging technologies of big data processing and analytics, such as advanced machine learning and deep learning technologies based on high-performance computing infrastructure. The applications cover different organ levels (root, shoot and seed) of different crops (grains, soybean, maize, potato) at different growth environments (open field and controlled environments). With the contribution of more than 20 world-leading researchers in high-throughput crop phenotyping, the authors hope this book provides readers the needed information to understand the concept, gain the insides and create the innovation of high-throughput phenotyping technology.

This book is the first attempt for in-depth compilation of current knowledge on cisgenic crops and their  potential prospects as a sustainable substitute for the controversial genetically modified crops. Innovative methodologies for the development of cisgenic crops for disease resistance, improved nutritional contents, suitability for organic farming, survival under climate change, and their role in conservation of plant genetic resources have been highlighted. Combined with molecular markers and genome editing, an advanced approach for crop improvement is reported. The book has 14 chapters authored by globally leading experts on the subject. This book is useful to the students, teachers, researchers and policy planners working across the disciplines of classical plant breeding up to the recent genetically modified and genome edited crops.

This book is the first attempt for in-depth compilation of current knowledge on cisgenic crops and their  potential prospects as a sustainable substitute for the controversial genetically modified crops. Innovative methodologies for the development of cisgenic crops for disease resistance, improved nutritional contents, suitability for organic farming, survival under climate change, and their role in conservation of plant genetic resources have been highlighted. Combined with molecular markers and genome editing, an advanced approach for crop improvement is reported. The book has 14 chapters authored by globally leading experts on the subject. This book is useful to the students, teachers, researchers and policy planners working across the disciplines of classical plant breeding up to the recent genetically modified and genome edited crops.

This book is the first comprehensive compilation of existing knowledge on the impact of cisgenic crops on biodiversity, environment, and ecosystem. Strategies to create and access cisgenic crops, potential risks assessment, and legal implications across the globe (European Union, North and South Americas, Africa, and Asia) are enumerated. Ethics, economics, safety, social concerns, and consumer acceptance of cisgenic foods are deliberated. Policy paradigm and ways to overcome GM regulations through cisgenic crops are highlighted. The book has 12 chapters authored by internationally leading experts on the subject. This book will be useful especially for the policy makers and GMO regulators, while the students, teachers, and researchers from across the globe will be equally benefited.

This book is the first comprehensive compilation of existing knowledge on the impact of cisgenic crops on biodiversity, environment, and ecosystem. Strategies to create and access cisgenic crops, potential risks assessment, and legal implications across the globe (European Union, North and South Americas, Africa, and Asia) are enumerated. Ethics, economics, safety, social concerns, and consumer acceptance of cisgenic foods are deliberated. Policy paradigm and ways to overcome GM regulations through cisgenic crops are highlighted. The book has 12 chapters authored by internationally leading experts on the subject. This book will be useful especially for the policy makers and GMO regulators, while the students, teachers, and researchers from across the globe will be equally benefited.

This book presents a detailed overview and critical evaluation of recent advances and remaining challenges in improving nutritional quality and/or avoiding the accumulation of undesirable substances in plants using a variety of strategies based on modern biological tools and techniques. Each review chapter provides an authoritative and insightful account of the various aspects of nutritional enhancement of plants. In the course of the last two decades, several food crops rich in macro- and micronutrients have been developed to improve health and protect a large section of the populace in developing countries from chronic diseases. Providing extensive information on these developments, this book offers a valuable resource for all researchers, students and industrialists working in agriculture, the plant sciences, agronomy, horticulture, biotechnology, food and nutrition, and the soil and environmental sciences.

This contributed volume offers a comprehensive exploration of the latest breakthroughs in the fields of transcriptomics and epitranscriptomics, with an emphasis on understanding gene expression and RNA modifications in plants. It introduces fundamental concepts, including RNA sequencing technologies used to investigate these biological processes. The book covers key topics such as cutting-edge techniques employed to decipher the transcriptome and epitranscriptome, as well as the roles of these subdisciplines in plant development, adaptation, stress responses, and other critical biological functions. It also discusses emerging technologies and challenges in the field, as well as prospects for research and development.This book is essential for researchers in plant genetics, plant molecular biology, and biotechnology. It also serves as excellent reading material for undergraduate and graduate students seeking to understand the field of transcriptomics.

This book offers an in-depth exploration of non-coding RNA and its critical role in enhancing plant abiotic stress tolerance. It provides a comprehensive overview of non-coding DNA's structural and functional diversity and its regulatory mechanisms in stress responses.The chapters cover topics such as epigenetic regulation, mobile genetic elements, and genomic profiling techniques. It covers latest research on non-coding RNA-mediated regulation, transcriptional regulatory elements controlling stress-responsive gene expression, and the role of long non-coding RNAs (lncRNAs) in fine-tuning gene expression networks under stress conditions. The book also discusses chromatin modifications, transposon-mediated regulation, high-throughput sequencing approaches for identifying stress-responsive ncRNAs, and CRISPR-based functional analysis. In addition to exploring these advanced topics, the book highlights practical implications for crop improvement strategies.Researchers in plant biology, genomics, molecular biology, and agricultural sciences will find this book particularly beneficial. It is also an essential resource for educators looking to provide their students with up-to-date information on cutting-edge research techniques. By offering both theoretical perspectives and practical case studies from renowned experts worldwide, this volume equips readers with actionable knowledge to address real-world challenges in agriculture.

This edited book is a comprehensive compilation of applications of plant molecular farming in various fields including agriculture, industry and medicine, using different plant/crop systems as bioreactors for the bulk production of products of interest. The book places special emphasis on plant molecular farming being utilized for the bulk production of biologics, industrial enzymes, vaccines, drugs and recombinant pharmaceutical proteins.Plant molecular farming (PMF) is an upcoming promising interdisciplinary subject, and therefore the book contains contributions from experts across the disciplines. The utilization of plant viral vectors to produce biologics, virus-like particles and antimicrobial peptides via PMF have been highlighted. Molecular farming for the bulk production of recombinant pharmaceutical proteins and various types of human and veterinary vaccines in different crops/plants and genetically modified seeds has been included. The process of scaling up to manufacturing capacity, product formation by bioprocessing of plant cell suspension culture, and bioreactor engineering have been incorporated. Lastly, the book provides special coverage on utilizing PMF made products to control neglected tropical diseases and plant-made antigens as immunotherapies for allergic and autoimmune diseases.The book has chapters from leading experts on the subject, making it equally beneficial for researchers, policy planners, industrialists, medical professionals, entrepreneurs, and students of various related disciplines.

This edited book is a comprehensive compilation of applications of plant molecular farming in various fields including agriculture, industry and medicine, using different plant/crop systems as bioreactors for the bulk production of products of interest. The book places special emphasis on plant molecular farming being utilized for the bulk production of biologics, industrial enzymes, vaccines, drugs and recombinant pharmaceutical proteins.Plant molecular farming (PMF) is an upcoming promising interdisciplinary subject, and therefore the book contains contributions from experts across the disciplines. The utilization of plant viral vectors to produce biologics, virus-like particles and antimicrobial peptides via PMF have been highlighted. Molecular farming for the bulk production of recombinant pharmaceutical proteins and various types of human and veterinary vaccines in different crops/plants and genetically modified seeds has been included. The process of scaling up to manufacturing capacity, product formation by bioprocessing of plant cell suspension culture, and bioreactor engineering have been incorporated. Lastly, the book provides special coverage on utilizing PMF made products to control neglected tropical diseases and plant-made antigens as immunotherapies for allergic and autoimmune diseases.The book has chapters from leading experts on the subject, making it equally beneficial for researchers, policy planners, industrialists, medical professionals, entrepreneurs, and students of various related disciplines.

This edited volume is focused on genomic study of tea crop. This book includes 20 chapters that cover the most relevant and hot topics in tea plant genetics and genomics. A first set of chapters includes its global economic and healthy importance, the botany and taxonomy, main quality and functional components. A second group of chapters deals with genetics, breeding and includes genetic resources, commercial breeding, genetic transformation techniques, as well as the use of marker assisted selection (QTL, GWAS). This will be followed by a set of chapters on omics, including the genomics, transcriptomics, metabolomics, proteomics, organelle genome, small RNA and DNA methylation. Two chapters are devoted to biotic and abiotic stresses, continued by two others more chapters focused on the SNP array, and databases for molecular design breeding. Finally, a chapter deals with future perspectives in the omics era for tea breeding.The tea plant is a cross-pollinated, self-incompatible, high heterozygosity, very large genome (~3.2 Gb) which have greatly hindered research and breeding in this crop. In the recent years, modern genetic and genomic tools have contributed to the development of significant valuable resources for the tea genetic improvement.This book is of interest to teachers, tea researchers, tea breeders and tea lovers. Also, the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, horticulture, beverage plant sciences.

This edited volume is focused on genomic study of tea crop. This book includes 20 chapters that cover the most relevant and hot topics in tea plant genetics and genomics. A first set of chapters includes its global economic and healthy importance, the botany and taxonomy, main quality and functional components. A second group of chapters deals with genetics, breeding and includes genetic resources, commercial breeding, genetic transformation techniques, as well as the use of marker assisted selection (QTL, GWAS). This will be followed by a set of chapters on omics, including the genomics, transcriptomics, metabolomics, proteomics, organelle genome, small RNA and DNA methylation. Two chapters are devoted to biotic and abiotic stresses, continued by two others more chapters focused on the SNP array, and databases for molecular design breeding. Finally, a chapter deals with future perspectives in the omics era for tea breeding.The tea plant is a cross-pollinated, self-incompatible, high heterozygosity, very large genome (~3.2 Gb) which have greatly hindered research and breeding in this crop. In the recent years, modern genetic and genomic tools have contributed to the development of significant valuable resources for the tea genetic improvement.This book is of interest to teachers, tea researchers, tea breeders and tea lovers. Also, the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, horticulture, beverage plant sciences.

This volume presents detailed reviews on biochemistry, classical genetics and traditional breeding, germplasm diversity, molecular genetics, tissue culture, biotechnology, and structural and functional genomics of ten major seed spices including ajwain, anise, celery, coriander, cumin, dill, fennel, fenugreek, Nigella and parsely.The seed spices include the most ancient crops of the world being cultivated for thousands of years as spices, condiments and herbal medicines. Besides adding attractive flavor and taste, seed spices are used as preservatives, and preventive and curative phytomedicines. Despite the immense importance of these crops and their multipurpose utilities, there is no comprehensive compilation of the information on genetics, genomics and breeding in these crops. The present book fills up that gap in literature.This book is useful to the students, teachers and scientists in the academia and relevant scientists interested in genetics, genomics, breeding and genetic engineering and also crop production, pathology, physiology, of seed spices. This book is also useful to the seed companies and industries engaged in long-term preservation, essential oil-based products and pharmaceuticals.

This volume presents detailed reviews on biochemistry, classical genetics and traditional breeding, germplasm diversity, molecular genetics, tissue culture, biotechnology, and structural and functional genomics of ten major seed spices including ajwain, anise, celery, coriander, cumin, dill, fennel, fenugreek, Nigella and parsely.The seed spices include the most ancient crops of the world being cultivated for thousands of years as spices, condiments and herbal medicines. Besides adding attractive flavor and taste, seed spices are used as preservatives, and preventive and curative phytomedicines. Despite the immense importance of these crops and their multipurpose utilities, there is no comprehensive compilation of the information on genetics, genomics and breeding in these crops. The present book fills up that gap in literature.This book is useful to the students, teachers and scientists in the academia and relevant scientists interested in genetics, genomics, breeding and genetic engineering and also crop production, pathology, physiology, of seed spices. This book is also useful to the seed companies and industries engaged in long-term preservation, essential oil-based products and pharmaceuticals.

This edited book is an in-depth compilation of recent tools and techniques, concepts and strategies used globally in plant molecular farming (PMF) for the cost-effective bulk production of recombinant proteins, secondary metabolites, and other biomolecules. The book presents an overview of success stories of PMF applications from developing countries to address poverty, achieve zero hunger, good health and well-being, thus achieving the UN SDGs 1, 2, and 3.The book deep dives into recent extraction and downstream processing methodologies, its co-existence with conventional agriculture, global governance and finally opportunities, challenges, and future perspectives in plant molecular farming. It focuses on plastid/chloroplast transformation (transplastomics) and its application in plant molecular farming. The books highlight recent advances in genome editing, synthetic biology, glycosylation and glyco-engineering for improved plant molecular farming by marker-free andtissue-specific systems via cisgenic and transgenic crops. In depth discussions on biosafety issues and bio-containment strategies have also been included.The book has 15 chapters authored by globally leading experts on the subject, presenting opportunities & challenges for bio-industrial researchers and entrepreneurs. It is useful to researchers, industrialists, entrepreneurs, policy planners, academician, and students across the disciplines.