Recombinant Antibodies for Cancer Therapy

"...the book is well written and the experimental protocols that were included are described in great detail...this book is a valuable resource both for persons new to this field to get an overview of current activities and for those already in the field to look up specific areas that are covered." -Clinical Immunology "Overall the book is a helpful resource, especially for molecular biologists involved in generating antibody fragments, collecting up to date protocols in one convenient volume." - Pharmaceutical Research

• Generation of Antibody Molecules Through Antibody Engineering.- Application of Recombinant Antibodies in Cancer Patients.- Hybridoma-Derived Antibodies.- DNA Immunization as a Means to Generate Antibodies to Proteins.- Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma.- Humanization of Monoclonal Antibodies by CDR Grafting.- Recombinant Antibody Fragments from Phagemid-Displayed Antibody Repertoires.- Generation and Screening of a Modular Human scFv Expression Library from Multiple Donors.- Construction of Semisynthetic Antibody Libraries.- Single-Domain VH Antibody Fragments from a Phage Display Library.- Isolation of Human Fab Fragments Against Ovarian Carcinoma Using Guided Selection.- Human Recombinant Fab Antibodies with T-Cell Receptor-Like Specificities Generated from Phage Display Libraries.- Engineering Hot Spots for Affinity Enhancement of Antibodies.- Simultaneous Humanization and Affinity Optimization of Monoclonal Antibodies.- Tailoring Kinetics of Antibodies Using Focused Combinatorial Libraries.- Antibody Fragments with Additional Properties.- Engineering scFvs for Improved Stability.- Recombinant Single-Chain and Disulfide-Stabilized Fv Immunotoxins for Cancer Therapy.- Generation of Recombinant Immunotoxins for Specific Targeting of Tumor-Related Peptides Presented by MHC Molecules.- Construction and Characterization of RNase-Based Targeted Therapeutics.- Bispecific Diabodies for Cancer Therapy.- Generation and Characterization of Bispecific Tandem Diabodies for Tumor Therapy.- Generation of Recombinant Multimeric Antibody Fragments for Tumor Diagnosis and Therapy.- Construction and Characterization of Minibodies for Imaging and Therapy of Colorectal Carcinomas.- Generation, Expression, and Monitoring of Recombinant Immune Receptorsfor Use in Cellular Immunotherapy.- Tailoring Natural Effector Functions.- Single-Chain Fv-Based Affinity Purification of the Cellular Stress Protein gp96 for Vaccine Development.- Recombinant Adenoviruses for In Vivo Expression of Antibody Fragments.- Large Scale Production of Recombinant Antibodies for Clinical Application.- Production of Antibody Fragments in a Bioreactor.- Large Scale Production of Recombinant Antibodies by Utilizing Cellulose-Binding Domains.- Production of Tumor-Specific Antibodies in Tobacco.