This detailed volume presents a comprehensive methodological guide for the study of chimeric RNAs, from their discovery in high-throughput datasets to their validation in the lab and characterization in biological and clinical contexts. Beginning with chapters dedicated to the identification and characterization of chimeric RNAs using numerous bioinformatic tools, this book continues by covering experimental validation and quantification of chimeric RNAs, methods for chimeric RNA functional and mechanistic studies, and translational application of chimeric RNAs and case studies. Written for the highly successful Methods in Molecular Biology series, chapters generally include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Chimeric RNAs: Methods and Protocols, Second Edition serves as an ideal guide to the practical guidance and conceptual grounding needed to advance your work in this increasingly vital area of study.
