Methods in Gut Microbial Ecology for Ruminants

AvHarinder P.S. Makkar,Christopher S. McSweeney

Inbunden, Engelska, 2005

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Beskrivning

Asaresultofvarioushumanactivities,suchasincreaseinhumanpopulation,decrease in arable land due to soil degradation, urbanization, industrialization and associated increase in the demand for livestock products, dramatic changes are occurring in the global ruminant livestock sector.

Produktinformation

Utgivningsdatum:2005-11-03
Mått:156 x 232 x 17 mm
Vikt:527 g
Format:Inbunden
Språk:Engelska
Antal sidor:226
Upplaga:2005
Förlag:Springer-Verlag New York Inc.
ISBN:9781402037900

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Innehållsförteckning

Foreword; Introduction; Editorial Note; Part 1. Designing in-vivo Microbial Ecology Studies; 1.1. Experimental designs for rumen microbiology, Adrian R. Egan; Part 2. Classical Methods for Isolation, Enumeration, Cultivation and Functional Assays of Rumen Microbes; 2.1. Rumen bacteria, Christopher S. McSweeney, Stuart E. Denman and Roderick I. Mackie; 2.2. Bacteriophages, Athol V. Klieve. 2.3. Methanogenic archaea, Keith N. Joblin; 2.4. Anaerobic fungi, Michael K. Theodorou, Jayne Brookman and Anthony P.J. Trinci; 2.5. Ciliate protozoa, Burk A. Dehority; Part 3. PCR-Based Methods for Analysis of Populations and Gene Expression; 3.1. Nucleic acid extraction, oligonucleotide probes and PCR methods, Zhongtang Yu and Robert J. Forster; 3.2. Quantitative (real-time) PCR, Stuart E. Denman and Christopher S. McSweeney; Part 4. Molecular Fingerprinting Techniques for Genotypic Analysis of Pure Cultures and Microbial Communities; 4.1. Denaturing gradient gel electrophoresis, Svetlana A. Kocherginskaya, Isaac K.O. Cann and Roderick I. Mackie; 4.2. Bacteriophage populations, Athol V. Klieve and Rosalind A. Gilbert; 4.3. Anaerobic fungal populations, Jayne L. Brookman and Matthew J. Nicholson; 4.4. RAPD, RFLP, T-RFLP, AFLP, RISA, Stuart E. Denman, Makoto Mitsumori and Christopher S. McSweeney; Part 5. DNA Clone Libraries of Microbial Communities; 5.1. 16S/18S ribosomal DNA clone library analysis of rumen microbial diversity, André-Denis G. Wright, Kiyoshi Tajima and Rustam I. Aminov; Part 6. Use of Small Subunit Ribosomal RNA Directed Oligonucleotide Probes for Microbial Population Studies; 6.1. Northern blot analysis to investigate the abundance of micro-organisms, Denis O. Krause; 6.2. Whole cell probing with fluorescently labelled probes for in situ analysis of microbial populations, Linda L. Blackall; 6.3. Combined fluorescence in situ hybridization and microautoradiography (FISH-MAR), Maneesha P. Ginige; Part 7. Genomic Analysis of Microbial Ecosystems; 7.1.Metagenomic analysis of the microbiomes in ruminants and other herbivores Mark Morrison, Sarah E. Adams, Karen E. Nelson and Graeme T. Attwood; Chapterwise Keywords; Keyword Index.