Peixuan Guo, Ph. D. William Farish Endowed Chair in Nano biotechnology Professor of School of Pharmacy Director of Nano biotechnology Center University of Kentucky Director of NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy Mailing address College of Pharmacy University of Kentucky 565 Biopharm Complex 789 S. Limestone Street Lexington, KY 40536 Phone: (Office) (859)218-0128 (Cell Phone): (513)728-1411 peixuan.guo@uky.edu (future)

• Overview of Methods in RNA Nanotechnology - Synthesis, Purification and Characterization of RNA Nanoparticles.- Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-Assembly.- Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM.- Silver Nano clusters for RNA Nano technology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies.- Purification of RNA Nanoparticles by Ultracentrifugation.- HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single Nucleotide Resolution.- RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover In Vivo.- Fluorescence Labeling of Short RNA by Oxidation at the 3’-End.- Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases.- Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor.- Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide-Protein Complexes to SELEX Screens.- Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge.- A High Throughput Screening Assay for the Functional Delivery of Splice-Switching Oligonucleotides in Human Melanoma Cells.- Design, Assembly, and Evaluation of RNA-Protein Nanostructures.- Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq.- Mapping Protein-RNA Interactions by RCAP, RNA-Crosslinking and Peptide Fingerprinting.

Peixuan Guo, Ph. D. William Farish Endowed Chair in Nano biotechnology Professor of School of Pharmacy Director of Nano biotechnology Center University of Kentucky Director of NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy Mailing address College of Pharmacy University of Kentucky 565 Biopharm Complex 789 S. Limestone Street Lexington, KY 40536 Phone: (Office) (859)218-0128 (Cell Phone): (513)728-1411 peixuan.guo@uky.edu (future)

• Overview of Methods in RNA Nanotechnology - Synthesis, Purification and Characterization of RNA Nanoparticles.- Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-Assembly.- Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM.- Silver Nano clusters for RNA Nano technology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies.- Purification of RNA Nanoparticles by Ultracentrifugation.- HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single Nucleotide Resolution.- RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover In Vivo.- Fluorescence Labeling of Short RNA by Oxidation at the 3’-End.- Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases.- Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor.- Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide-Protein Complexes to SELEX Screens.- Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge.- A High Throughput Screening Assay for the Functional Delivery of Splice-Switching Oligonucleotides in Human Melanoma Cells.- Design, Assembly, and Evaluation of RNA-Protein Nanostructures.- Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq.- Mapping Protein-RNA Interactions by RCAP, RNA-Crosslinking and Peptide Fingerprinting.