C. elegans

Methods and Applications

AvDavid Biron,Gal Haspel

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

1 202 kr

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Beskrivning

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, C.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 15 mm
Vikt:503 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:252
Upplaga:2
Förlag:Humana Press Inc.
ISBN:9781493948925

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik
Medicinsk genetik inom Medicin

Innehållsförteckning

Library Construction for Mutation Identification by Whole-Genome Sequencing.- Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes.- Genetic Methods for Cellular Manipulations in C. elegans.- A Fusion PCR Method for Expressing Genetic Tools in C. elegans.- Transposon Assisted Genetic Engineering with Mos1 Mediated Single-copy Insertion (MosSCI).- Creating Genome Modifications in C. elegans using the CRISPR/Cas9 System.- Observing and Quantifying Fluorescent Reporters.- Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior.- Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans.- High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegans.- Electron Tomography Methods for C. elegans.- Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans.- Tracking Single C. elegans using a USB Microscope on a Motorized Stage.- An Imaging System for C. elegans Behavior.- A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans dauer Larvae.- Sampling and Isolation of C. elegans from the Natural Habitat.- A Primer on Prototyping.- A Primer on Quantitative Modeling.