TALENs

Methods and Protocols

AvRalf Kühn,Wolfgang Wurst

Häftad, Engelska, 2016

Del 1338 i serien Methods in Molecular Biology

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Beskrivning

Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions.

Produktinformation

Utgivningsdatum:2016-08-23
Mått:178 x 254 x 16 mm
Vikt:547 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:274
Förlag:Humana Press Inc.
ISBN:9781493949052

Utforska kategorier

Biovetenskap inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik
Medicinsk genetik inom Medicin

Innehållsförteckning

Xanthomonas and the TAL effectors: Nature's Molecular Biologist.- TAL Effector DNA-binding Principles and Specificity.- The Development of TALE Nucleases for Biotechnology.- Online Tools for TALEN Design.- Assembly of Customized TAL Effectors through Advanced ULtiMATE System.- Engineering Customized TALENs Using the Platinum Gate TALEN Kit.- Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors.- A New Approach to Dissect Nuclear Organization; TALE-mediated Genome Visualization (TGV).- TALEN Induced Translocations in Human Cells.- Mutagenesis in Newts; Protocol for Iberian Ribbed Newts.- Targeted Mutagenesis in Bombyx mori Using TALENs.- GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, The TwoSpotted Cricket Gryllus bimaculatus, using TALENs.- Methods for TALEN Evaluation, use, and Mutation Detection in the Mosquito Aedes aegypti.- Methods for TALEN-mediated Genomic Manipulations in Drosophila.- Targeted Mutagenesis in Zebrafish by TALENs.- Mutagenesis in Xenopus and Zebrafish using TALENs.- Genome Editing in Mice using TALE Nucleases.- Genome Editing in Rats using TALE Nucleases.- Designer Nuclease-mediated Generation of Knock out THP1 Cells.