Cell-free Production

Yuan Lu is an Associate Professor at the Department of Chemical Engineering, Tsinghua University, China. After receiving B.S. (2004) and Ph.D. (2009) in chemical engineering from Tsinghua University, China, Dr Lu received postdoctoral training from Johns Hopkins University (2009-2010) and Stanford University (2010-2014), USA. He was a project researcher from 2014 to 2016 at the University of Tokyo, Japan. The central paradigm of Dr Lu’s research is using interdisciplinary approaches in engineering, biology, chemistry, physics, and computer science to manipulate biomolecules and complex biological systems. The main focus lies on developing and applying synthetic biology and other cutting-edge technologies to engineer nucleic acids, proteins, pathways, and networks for addressing most daunting challenges in human health.Michael Jewett is the Walter P. Murphy Professor of Chemical and Biological Engineering and Director of the Center for Synthetic Biology at Northwestern University, USA. Dr Jewett received his PhD in 2005 from Stanford University, USA, completed postdoctoral studies at the Center for Microbial Biotechnology in Denmark and the Harvard University Medical School, and was a guest professor at the Swiss Federal Institute of Technology (ETH Zurich). He is the recipient of the NIH Pathway to Independence Award, David and Lucille Packard Fellowship in Science and Engineering, Camille-Dreyfus Teacher-Scholar Award, and a Finalist for the Blavatnik National Awards for Young Scientists, among others. He is the co-founder of SwiftScale Biologics, Stemloop Inc., Pearl Bio, Induro Therapeutics, and Design Pharmaceuticals. Jewett is a Fellow of AIMBE, AAAS, and NAI. Rather than attempt to balance the tug-of-war that exists between the cell’s physiological and evolutionary objectives on one side and the engineer’s process objectives on the other, Dr Jewett’s group is developing new strategies that widen the aperture of the traditional model of biotechnology. In one direction, the group seeks to create a new paradigm for engineering biocatalytic systems using cell-free biology. The foundational principle is that one can conduct precise, complex biomolecular transformations in crude lysates without using intact cells. This concept represents a significant departure from cell-based processes that rely on microscopic cellular ‘reactors,’ and provides an unprecedented and otherwise unattainable freedom of design to modify and control biological systems. Dr Jeweet’s groups is also pioneering new directions to repurpose the translation apparatus for synthetic biology. The goal is to monitor, interrogate, and understand the process of translation, and with this knowledge diversify, evolve and repurpose the ribosome and its peripheral machinery into a re-engineered machine to generate non-natural polymers as new classes of sequence-defined, evolvable matter.

• Cell-free biomanufacturing by purified cascade multi-enzymes.- Hydrogel based multi-enzymatic system for biosynthesis.- Cofactor synthesis and regeneration for cell-free enzymatic cascades.- The evolution with cell-free system.- Cell-free synthetic biology at the interface between biology can chemistry.- Rapid and finely-tuned production for deployable sensing applications.- Transcriptional regulation in cell-free systems and artificial cells.- Cell-free synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes.- Compartmentalized cell-free expression systems for bottom-up synthetic biology.- Exploring the arcane – cell-free as a tool to study prokaryotic and eukaryotic systems.