This book presents the proceedings of the International Conference on Motion Assisting Devices for Limbs (ASSIST), held in Rome, Italy on November 19-20, 2025. Within the context of the project P2022A3ELB – PRIN 2022 PNRR, it includes peer-reviewed papers on motion assisting devices and modular arm/leg exoskeleton for motion assistance, discussing topics such as bio-inspired assisting devices, kinematics and dynamics, performance analysis, vision, control and motion planning, human-machine interfaces, new designs and prototypes, exoskeletons and service medical robots, testing and applications. A valuable, up-to-date resource, it offers an essential overview of the subject for scientists and practitioners alike, and will inspire further investigations and research.