Hirak Ranjan Dash – författare

Microbial Diversity in the Genomic Era presents insights on the techniques used for microbial taxonomy and phylogeny, along with their applications and respective pros and cons. Though many advanced techniques for the identification of any unknown bacterium are available in the genomics era, a far fewer number of the total microbial species have been discovered and identified to date. The assessment of microbial taxonomy and biosystematics techniques discovered and practiced in the current genomics era with suitable recommendations is the prime focus of this book.

Discusses the techniques used for microbial taxonomy and phylogeny with their applications and respective pros and cons Reviews the evolving field of bacterial typing and the genomic technologies that enable comparative analysis of multiple genomes and the metagenomes of complex microbial environments Provides a uniform, standard methodology for species designation

Microbial and Natural Macromolecules: Synthesis and Applications brings together active scientists and academicians in the field who share updated information and research outcomes from global experts. Microbial macromolecular diversity, molecular composure, genetics, usability of advanced molecular tools and techniques for their study as well as their applicability are discussed with detailed research perspectives.

Illustrates fundamental discoveries and methodological advancements Discusses novel functional attributes of macromolecules Updates progress on microbial macromolecular research

Microbial Biodegradation and Bioremediation: Techniques and Case Studies for Environmental Pollution, Second Edition describes the successful application of microbes and their derivatives for bioremediation of potentially toxic and relatively novel compounds in the environment. Our natural biodiversity and environment is in danger due to the release of continuously emerging potential pollutants by anthropogenic activities. Though many attempts have been made to eradicate and remediate these noxious elements, thousands of xenobiotics of relatively new entities emerge every day, thus worsening the situation. Primitive microorganisms are highly adaptable to toxic environments, and can reduce the load of toxic elements by their successful transformation and remediation.

This completely updated new edition presents many new technologies and techniques and includes theoretical context and case studies in every chapter. Microbial Biodegradation and Bioremediation: Techniques and Case Studies for Environmental Pollution, Second Edition serves as a single-source reference and encompasses all categories of pollutants and their applications in a convenient, comprehensive format for researchers in environmental science and engineering, pollution, environmental microbiology, and biotechnology.

Describes many novel approaches of microbial bioremediation including genetic engineering, metagenomics, microbial fuel cell technology, biosurfactants and biofilm-based bioremediation Introduces relatively new hazardous elements and their bioremediation practices including oil spills, military waste water, greenhouse gases, polythene wastes, and more Provides the most advanced techniques in the field of bioremediation, including insilico approach, microbes as pollution indicators, use of bioreactors, techniques of pollution monitoring, and more Completely updated and expanded to include topics and techniques such as genetically engineered bacteria, environmental health, nanoremediation, heavy metals, contaminant transport, and in situ and ex situ methods Includes theoretical context and case studies within each chapter

Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows.

With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies.

Describes the fundamentals of NGS and its advances for forensic applications Explains the transition from CE-based technique to NGS technology Includes case studies related to NGS and DNA fingerprinting Explores the future use and applications of NGS technologies

Around the World, metal pollution is a major problem. Conventional practices of toxic metal removal can be ineffective and/or expensive, delaying and exacerbating the crisis. Those communities dealing with contamination must be aware of the fundamentals advances of microbe-mediated metal removal practices because these methods can be easily used and require less remedial intervention. This book describes innovations and efficient applications for metal bioremediation for environments polluted by metal contaminates.

Microbial Diversity in the Genomic Era: Functional Diversity and Community Analysis, Second Edition presents techniques used for microbial taxonomy and phylogeny, along with their applications and respective strengths and challenges. The book incorporates recently developed biosystematics methods and approaches to assess microbial taxonomy, with suitable recommendations for where to apply them across the range of bacterial identification and infectious disease research. Sections provide a broad overview of microbial genomics research and microbiome directed medicine and update on molecular tools for microbial diversity research, extremophilic microbial diversity, functional microbial diversity across application areas, microbial diversity and infectious disease research, and future directions. Step-by-step methodologies are provided for key techniques, along with applied case studies that break down recent research studies into practical components, thus illuminating pathways for new studies across the field.Instructs in techniques used for microbial taxonomy and phylogeny, with discussions of their applications and respective pros and consReviews the evolving field of microbial typing and the genomic technologies that enable comparative metagenomic analysis of complex microbial environmentsCovers microbiome directed translational research, as well as whole genome sequencing for studying microbial diversity, with newly added research protocols and case studiesReviews future applications in the field of microbiome directed medicineFeatures chapter contributions from global experts in the field

Investigative and Predictive DNA Testing delves into the cutting-edge of DNA analysis, presenting a comprehensive guide that unlocks the potential of DNA in solving complex cases. This book stands as a vital resource for students, practitioners, and researchers in fields such as biology, zoology, medicine, anthropology, microbiology, and genetics. Designed for those with a basic understanding of biological sciences, it is particularly beneficial for graduate, post-graduate, and research level students. The book explores the evolution of DNA analysis, focusing on predictive methods and investigative leads such as ancestry, lineage, and phenotype.Each chapter, contributed by internationally renowned experts, highlights the transformative role of artificial intelligence and machine learning in enhancing the accuracy and utility of DNA analysis. The book is comprised of various sections, starting with fundamental concepts of investigative and predictive DNA analysis, followed by technological advancements in the field, and concluding with the challenges and future concerns of DNA analysis. It serves as an indispensable tool for anyone looking to expand their knowledge and skills in DNA analysis, making it a must-read for the scientific community.Describes the fundamentals of investigative and predictive approaches for forensic applicationsExplains the technological advances in forensic DNA analysisIncludes real case studies related to predictive DNA analysisProvides futuristic use and applications of predictive forensic DNA analysisUses real time images for easy understanding

Investigative and Predictive Forensic Microbiology showcases the latest advancements in microbial technology for forensic applications, covering a wide range of topics in forensic microbiology. Sections explore human normal microflora, cover classical methods of analyzing microbes, discuss microbial tools in forensic medicine that address post-mortem analysis, and delve into microbial biomarkers for estimating time of death. Investigative microbiology topics include analyzing body fluids, geolocation prediction using microbes, ancient microbial DNA analysis, biodefense, and emerging infections. The book's final section focuses on challenges and future approaches, covering statistical interpretation of data, regulatory considerations, and ethical implications of forensic microbiology.With contributions from renowned experts in the field, this book is a comprehensive resource for researchers and practitioners in microbiology, genetics, forensic science, and for law enforcement professionals and advanced students.Describes the fundamentals of forensic microbiologyExplains and summarizes technological advancements in forensic microbiologyIncludes examples and historic case studies related to the application of microbial forensics

The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples.It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples.Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis. Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications.

The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments.

Around the World, metal pollution is a major problem. Conventional practices of toxic metal removal can be ineffective and/or expensive, delaying and exacerbating the crisis. Those communities dealing with contamination must be aware of the fundamentals advances of microbe-mediated metal removal practices because these methods can be easily used and require less remedial intervention. This book describes innovations and efficient applications for metal bioremediation for environments polluted by metal contaminates.

Microorganisms play an important role in the maintenance of the ecosystem structure and function. Bacteria constitute the major part of the microorganisms and possess tremendous potential in many important applications from environmental clean up to the drug discovery. Much advancement has been taken place in the field of research on bacterial systems. This book summarizes the experimental setups required for applied microbiological studies. Important background information, representative results, step by step protocol in this book will be of great use to the students, early career researchers as well as the academicians. The book describes many experiments covering the basic microbiological experiments to the applications of microbial systems for advanced research. Researchers in any field who utilize bacterial systems will find this book very useful. In addition to microbiology and bacteriology, this book will also find useful in molecular biology, genetics, and pathology and the volume should prove to be a valuable laboratory resource in clinical and environmental microbiology, microbial genetics and agricultural research. Unique features • Easy to follow by the users as the experiments have been written in simple language and step-wise manner. • Role of each reagents to be used in each experiment have been described which will help the beginners to understand quickly and design their own experiment. • Each experiment has been equipped with the coloured illustrations for proper understanding of the concept. • Trouble-shootings at the end of each experiment will be helpful in overcoming the problems faced by the users. • Flow-chart of each experiment will quickly guide the users in performing the experiments.

Microorganisms play an important role in the maintenance of the ecosystem structure and function. Bacteria constitute the major part of the microorganisms and possess tremendous potential in many important applications from environmental clean up to the drug discovery. Much advancement has been taken place in the field of research on bacterial systems. This book summarizes the experimental setups required for applied microbiological studies. Important background information, representative results, step by step protocol in this book will be of great use to the students, early career researchers as well as the academicians. The book describes many experiments covering the basic microbiological experiments to the applications of microbial systems for advanced research. Researchers in any field who utilize bacterial systems will find this book very useful. In addition to microbiology and bacteriology, this book will also find useful in molecular biology, genetics, and pathology and the volume should prove to be a valuable laboratory resource in clinical and environmental microbiology, microbial genetics and agricultural research. Unique features • Easy to follow by the users as the experiments have been written in simple language and step-wise manner. • Role of each reagents to be used in each experiment have been described which will help the beginners to understand quickly and design their own experiment. • Each experiment has been equipped with the coloured illustrations for proper understanding of the concept. • Trouble-shootings at the end of each experiment will be helpful in overcoming the problems faced by the users. • Flow-chart of each experiment will quickly guide the users in performing the experiments.

This book describes the basics and various applications of DNA fingerprinting, including in actual case studies. Module 1: Basics of DNA Fingerprinting, Module 2: Applications of DNA Fingerprinting, Module 3: DNA Fingerprinting: Case Studies, and Module 4: Future of DNA Fingerprinting.

This book describes the basics and various applications of DNA fingerprinting, including in actual case studies. Module 1: Basics of DNA Fingerprinting, Module 2: Applications of DNA Fingerprinting, Module 3: DNA Fingerprinting: Case Studies, and Module 4: Future of DNA Fingerprinting.

The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis.Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis.Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science.Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics.The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping.Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level.

The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis.Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis.Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science.Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics.The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping.Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level.

This reference book comprehensively reviews the significance of DNA technology in forensic science. After presenting the theory, basic principles, tools and techniques that are used in forensic DNA typing, it summarizes various techniques, including autosomal STR, Y-STR, X-STR, mitochondrial DNA and NGS, used in solving both criminal as and civil cases, such as paternity disputes, identification of mutilated remains, and culprit identification in sexual assault and murder cases. It also provides an overview of DNA-based genetic diagnostics for various diseases, and discusses the role of DNA typing in drug reactions, as well as the application of non-human DNA profiling of animals and plants in forensic science investigations. Lastly, the book examines the role of internal quality control in maintaining the high quality of DNA profiling.

Forensic Investigation of Blood Evidence offers a comprehensive exploration of the scientific, investigative, and legal dimensions of blood as forensic evidence. From the moment blood is released at a crime scene, it becomes a critical biological marker—capable of revealing not only the presence of injury but also the identity of those involved. This book guides the reader through every stage of forensic blood analysis: from presumptive and confirmatory testing to species identification, blood grouping, DNA profiling, and bloodstain pattern interpretation. Emphasizing both foundational principles and cutting-edge techniques, it bridges traditional methodologies with innovations such as microbiome analysis and the application of artificial intelligence in forensic examination.Structured into four well-defined modules, the book delves into the biological and forensic underpinnings of blood, outlines conventional and advanced analytical strategies, and contextualizes real-world applications through crime scene reconstruction and case studies. Written in clear, accessible language, it serves as a vital resource for students, researchers, forensic practitioners, investigators, and legal professionals. Whether one seeks to understand the science behind blood evidence or its impact in courtroom proceedings, this book provides a vital and engaging reference grounded in both scientific rigor and practical insight.

The book explores the interdisciplinary field that merges biochemistry, cell biology, molecular biology, and genetics to unravel the mysteries of biological evidence in forensic investigations.

This textbook for undergraduate and postgraduate students discusses advancements in forensic DNA analysis since early texts were published.