cDNA Library Protocols

This is a good overview of the essentials for preparing a cDNA library, for both the novice and the expert...the book is a true laboratory manual for the experimental scientist. It Should represent a useful and up-to-date addition for any lab that, for whatever reason, needs to generate its own libraries.-Hoffman-LaRoche, Inc. "...their fine layout and ease of use suggest that the lab copy won't stay on the bookshelf for long."-SGM Quarterly

• cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR.- Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations.- Isolation of Messenger RNA from Plant Tissues.- cDNA Library Construction for the Lambda ZAP®-Based Vectors.- Clone Excision Methods for the Lambda ZAP®-Based Vectors.- Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs.- Inverse PCR Approach to Cloning cDNA Ends.- Cloning Gene Family Members Using the Polymerase Chain Reaction with Degenerate Oligonucleotide Primers.- Subtractive Hybridization for the Isolation of Differentially Expressed Genes Using Magnetic Beads.- Preparation of Competent Cells for High-Efficiency Plasmid Transformation of Escherichia coli.- The Tetramethylammonium Chloride (TMAC) Method for Screening cDNA Libraries with Highly Degenerate Oligonucleotide Probes Obtained by Reverse Translation of Amino Acid Sequences.- Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides.- Immunological Screening of ?-Phage cDNA Expression Libraries.- Cloning Sequence-Specific DNA-Binding Factors from cDNA Expression Libraries Using Oligonucleotide Binding Site Probes.- Protein Interaction Cloning by Far-Western Screening of ?-Phage cDNA Expression Libraries.- Yeast Two-Hybrid Library Screening.- Signal Sequence Trap.- Isolation of Genetic Suppressor Elements (GSEs) from Random Fragment cDNA Libraries in Retroviral Vectors.- Expression and Preparation of Fusion Proteins from Recombinant ?gt1.1 Phages.- Computer Analysis of Cloned Sequences.- The TIGR Human cDNA Database.- Searching the dbEST Database.- Reference cDNA Library Facilities Available from European Sources.