Transgenic Wheat, Barley and Oats

Production and Characterization Protocols

AvHuw D. Jones,Peter R. Shewry

Häftad, Engelska, 2016

Del i serien Methods in Molecular Biology

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Beskrivning

v Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Produktinformation

Utgivningsdatum:2016-08-23
Mått:193 x 260 x 20 mm
Vikt:768 g
Format:Häftad
Språk:Engelska
Serie:Methods in Molecular Biology
Antal sidor:349
Förlag:Humana Press Inc.
ISBN:9781493956357

Utforska kategorier

Innehållsförteckning

Transgenic Wheat, Barley and Oats: Production and Characterization.- Transformation and Regeneration.- Selection of Transformed Plants.- Reporter Genes.- Biolistics Transformation of Wheat.- Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos.- Floral Transformation of Wheat.- Highly Efficient Agrobacterium-Mediated Transformation of Wheat Via In Planta Inoculation.- Barley Transformation Using Biolistic Techniques.- Barley Transformation Using Agrobacterium-Mediated Techniques.- Transformation of Oats and Its Application to Improving Osmotic Stress Tolerance.- Gene and Protein Expression.- Promoter Sequences for Defining Transgene Expression.- Down-Regulation of Gene Expression by RNA-Induced Gene Silencing.- Characterisation of Transgenic Plants.- Gene Insertion Patterns and Sites.- Fluorescent In Situ Hybridization to Detect Transgene Integration into Plant Genomes.- Establishing Substantial Equivalence: Transcriptomics.- Establishing Substantial Equivalence: Proteomics.- Establishing Substantial Equivalence: Metabolomics.- Design and Management of Field Trials of Transgenic Cereals.- GM Risk Assessment.- Conclusions.- Transgenic Wheat, Barley and Oats: Future Prospects.