Drugs of Abuse

"It describes in detail laboratory methods used by researchers working in the area of drug abuse and drug addiction. This is a unique book. Few books deal in detail with the methods underlying experiments. It will be of primary value to active investigators with active laboratories."-Doody's Health Sciences Book Review Journal

• Special Reviews.- The Temporal Sequence of Changes in Gene Expression by Drugs of Abuse.- Effects of Psychomotor Stimulants on Glutamate Receptor Expression.- Adult Neural Stem/Progenitor Cells in the Forebrain.- Neuroprotective Effect of Naloxone in Inflammation-Mediated Dopaminergic Neurodegeneration.- Neuropharmacology and Neurotoxicity of 3,4-Methylenedioxymethamphetamine.- Learning and Memory Mechanisms Involved in Compulsive Drug Use and Relapse.- From Drugs of Abuse to Parkinsonism: The MPTP Mouse Model of Parkinson’s Disease.- Detection of MRNA Expression in the Striatum.- In Situ Hybridization with Isotopic Riboprobes for Detection of Striatal Neuropeptide mRNA Expression After Dopamine Stimulant Administration.- Combining In Situ Hybridization with Retrograde Tracing and Immunohistochemistry for Phenotypic Characterization of Individual Neurons.- Analysis of mRNA Expression Using Double In Situ Hybridization Labeling with Isotopic and Nonisotopic Probes.- Quantification of mRNA in Neuronal Tissue by Northern Analysis.- Analysis of Gene Expression in Striatal Tissue by Multiprobe RNase Protection Assay.- Analysis of mRNA Expression in Striatal Tissue by Differential Display Polymerase Chain Reaction.- Semiquantitative Real-Time PCR for Analysis of mRNA Levels.- Application of TaqMan RT-PCR for Real-Time Semiquantitative Analysis of Gene Expression in the Striatum.- Systematic Screening of Gene Expression Using a cDNA Macroarray.- Detection of Protein Expression in the Striatum.- Quantification of Protein in Brain Tissue by Western Immunoblot Analysis.- Immunohistochemical and Immunocytochemical Detection of Phosphoproteins in Striatal Neurons.- Analysis of Protein Expression in Brain Tissue by ELISA.- Dopamine Receptor Binding and QuantitativeAutoradiographic Study.- Analysis of DNA-Binding Activity in Neuronal Tissue with the Electrophoretic Mobility-Shift Assay.- Gene Function Analysis.- Viral-Mediated Gene Transfer to Study the Behavioral Correlates of CREB Function in the Nucleus Accumbens of Rats.- Generating Gene Knockout Mice for Studying Mechanisms Underlying Drug Addiction.- Antisense Approaches in Analyzing the Functional Role of Proteins in the Central Nervous System.- Striatal Culture Preparation.- Primary Striatal Neuronal Culture.- Primary Rat Mesencephalic Neuron-Glia, Neuron-Enriched, Microglia-Enriched, and Astroglia-Enriched Cultures.- Primary Culture of Adult Neural Progenitors.- Organotypic Culture of Developing Striatum.- Detection of Transmitter Release in the Striatum.- Microdialysis Coupled with Electrochemical Detection.- HPLC/EC Detection and Quantification of Acetylcholine in Dialysates.- Real-Time Measurements of Phasic Changes in Extracellular Dopamine Concentration in Freely Moving Rats by Fast-Scan Cyclic Voltammetry.- Measurement of Dopamine Uptake in Neuronal Cells and Tissues.- Behvioral Assessment and Others.- Laboratory Analysis of Behavioral Effects of Drugs of Abuse in Rodents.- Conditioned Place Preference.- Detection of Cell Proliferation and Cell Fate in Adult CNS Using BrdU Double-Label Immunohistochemistry.