Proteomic Methods in Neuropsychiatric Research

Inbunden, Engelska, 2017

Del 974 i serien Advances in Experimental Medicine and Biology

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Beskrivning

Due to continuous technical developments and new insights into the high complexity of neurological diseases, there is an increasing need for the application of proteomic technologies which can yield potential biomarker readouts for improved clinical management as well as for the development of new drugs by struggling pharmaceutical companies.

Produktinformation

Utgivningsdatum:2017-04-04
Mått:155 x 235 x 27 mm
Vikt:740 g
Format:Inbunden
Språk:Engelska
Serie:Advances in Experimental Medicine and Biology
Antal sidor:370
Förlag:Springer International Publishing AG
ISBN:9783319524788

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik
Medicinsk genetik inom Medicin

Innehållsförteckning

Preface – Proteomic studies in psychiatric disease and neurological disorders.- Part 1: REVIEWS.- Application of proteomic techniques for improved stratification and treatment of schizophrenia patients.- Multiplexing biomarker methods, proteomics and considerations for Alzheimer’s disease.- What have proteomic studies taught us about novel drug targets in autism?.- Application of proteomic approaches to accelerate drug development for psychiatric disorders.- Proteomic biomarker identification in cerebrospinal fluid for leptomeningeal metastases with neurological complications.- Connecting brain proteomics with behavioural neuroscience in translational animal models of neuropsychiatric disorders.- LC-MSE for qualitative and quantitative proteomicstudies of psychiatric disorders.- The utility of multiplex assays for identification of proteomic signatures in psychiatry.- Part 2: PROTOCOLS.- Blood sampling and preparation procedures for proteomic biomarker studies of psychiatric disorders.- Multiplex immunoassay profiling of serum in psychiatric disorders.- Sequential immunopreciptation of secretory vesicle proteins from biosynthetically-labelled cells.- 2d gel electrophoresis of insulin secretory granule proteins from biosynthetically-labelled pancreatic islets.- Two-dimensional gel electrophoresis: a reference protocol.- A two-dimensional difference gel electrophoresis (2D-DIGE) protocol for studies of neural precursor cells.- Identifying biomarker candidates in the blood plasma or serum proteome.- Selective reaction monitoring mass spectrometry for quantitation of glycolytic enzymes in post-mortem brain samples.- A selected reaction monitoring mass spectrometry protocol for validation of proteomic biomarker candidates in studies of psychiatric disorders.- Application of iTRAQ shotgun proteomics for measurement of brain proteins in studies of psychiatric disorders.- Co-immunoprecipitation for deciphering protein interactomes.- Sequential co-immunopreciptation and immunoblot approach to determine oligomerisation of G-protein coupled receptors.- A protocol for producing the maternal low protein rat model: a tool for pre-clinical proteomic studies.- A clinical study protocol to identify serum biomarkers predictive of response to antipsychotics in schizophrenia patients.- Generation of the acute phencyclidine rat model for proteomic studies of schizophreniaA protocol for generation of a corticosterone model of psychiatric disorders.- MK-801-treated oligodendrocytes as a cellular model to study schizophrenia.- Combining patient-reprogrammed neural cells and proteomics as a model to study psychiatric disorders.- SILAC mass spectrometry profiling: a psychiatric disorder perspective.- Preparation of peripheral blood mononuclear cells (PBMCs) as a model for proteomic studies psychiatric disorders.- Proteomic profiling of skin fibroblasts as a model of schizophreniaProteomic profiling of the pituitary gland in studies of psychiatric disorders.- Development of an assay for measuring proprotein-conversion activity on a multiplex magnetic bead-based array platform.- Phenotyping multiple subsets of immune cells in situ in formalin-fixed, paraffin-embedded tissue sections.- Lab-on-a-chip proteomic assays for psychiatric disorders.- Development of a user-friendly app for testing blood coagulation status in schizophrenia patients.- Part 3:FUTURE PERSPECTIVES.- Proteomic approaches to enable point-of-care testing and personalized medicine for psychiatric disorders.