Biocatalysis in Green Solvents offers a pragmatic overview and instruction in biocatalysis and enzymology of green solvents for sustainable industries and medicine, running from concept to application. Here, international experts in the field discuss structure-function relationships of enzymes in ionic liquids (ILs) and examine how enzymes act as selective catalysts for fine biochemical synthesis in non-aqueous environments. Several integral green biochemical processes of biocatalytic transformation and pure product separation are described in detail.

Application focused chapters discuss the role of biocatalysis in creating and implementing deep eutectic solvents, biomass derived solvents, sub and supercritical fluids, carbon dioxide biphasic systems, and enzymatic membrane reactors, as well as applying these biocatalytic processes in drug discovery and production.

Examines the structure-function relationships of enzymes in ionic liquids and biocatalytic processes for various applications across industry and medicine Includes clear instruction in core green biochemical processes-of (bio)catalytic transformation-and pure product separation Features chapter contributions from international experts across academia and industry

Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes.

Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.

Provides a thorough overview of biocatalyst and enzyme immobilization for research and practical application Presents methods based content that instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization Features chapter contributions from international leaders in the field

Biosurfactants: Research and Development provides a thorough overview of biosurfactant research and development across a range of settings and industries, highlighting the novel use of enzymes, metabolic and genetic engineering in biosurfactant production and showcasing diverse experimental models and approaches. Sections discuss fundamental characteristics of biosurfactants, their physio-chemical properties, and their differences from chemically synthesize surfactants, different research approaches for the study of known biosurfactants, and the genetic manipulation of microorganisms to increase biosurfactant productivity, or to produce molecules with improved characteristics.

Throughout the book, methods and approaches are discussed in easy-to-digest formats, with methods discussed, ranging from in silico approaches to classical biocatalysis omics analysis and metabolic engineering.

Provides a through overview of biosurfactant enzymes and microorganisms used in biosurfactant production Features instruction in a wide range of research and development approaches, ranging from in silico techniques to classical biocatalysis omics analysis and metabolic engineering Features chapter contributions from international leaders in the field

Ureases: Foundations, Classes, and Applications provides a thorough, practical analysis of ureases-enzymes of growing relevance across a range of biotechnological applications and drug discovery. Unique in many aspects, ureases are one of the few enzymes to have nickel in their active sites. This book covers all aspects of this enzymatic class starting with foundational overview and then providing historical urease research and current state, from basic biochemistry to the use of ureases as hallmarks in enzymology, crystallography, and bioinorganic chemistry. The different classes of ureases, structurally diverse but chemically equivalent, are individually discussed.The multi-protein, multi-step activation of ureases (with chemical modification of residues, transport, and transfer of nickel ions) are examined in-depth, along with the catalytic mechanisms of ureolysis and its inhibitors. The final two sections of the book address multiple applications of ureases in health and biotechnology, respectively, going from gastric ulcer treatment to architectural uses in buildings and engineering. Future applications and next steps in research are also considered.

Considers fundamental aspects of urease biochemistry, ureolysis and urease inhibitorsDiscusses urease research across a range of applications, from drug discovery to biosensors, water purification, architecture and the food industryFeatures chapter contributions from international leaders in the field

Proteolytic enzymes are found in all life forms, where they catalyze peptide bond hydrolysis during essential biological processes. Proteases of various pathogens have also been shown to use endogenous protease inhibitors to regulate the activity of host proteolytic enzymes encountered during infection.Proteolytic Enzymes and their Inhibitors in Infectious Pathogens brings together key experts to provide a thorough discussion of proteolytic enzymes and their endogenous inhibitors found in parasites, bacteria, and viruses. The book focuses on the respective roles of the different catalytic classes of proteolytic enzymes and their protein-protease inhibitors at the host-infectious organism interface, and how this knowledge may inform drug and vaccine design. Following a foundational overview of proteases and macromolecular inhibitors of pathogenic organisms, authors discuss recent research on distinct classes of proteolytic enzymes in pathogens. Classes include aspartic proteases, cysteine proteases, metalloproteases, serine proteases, and threonine proteases with experts contributing chapters on distinct enzymes and inhibitors. New methods to identify protease substrates and inhibitors are also provided, with step-by-step protocols to advance new research and drive drug discovery.Discusses proteolytic enzymes of parasites, bacteria, and viruses arranged by peptide bond cleavage classes, for improved integration of information across the infectious organismsIncludes information on the structure and interaction with proteases of macromolecular protease inhibitors, and how these structures can be engineered to provide more potent and specific protease inhibitorsProvides instructions in methods and technologies to identify physiological protease substrates, their cleavage specificities, and inhibitorsFeatures rich illustrations and diagrams of core concepts, including informative three-dimensional protein structures and molecular interactions

Glycoside Hydrolases provides a detailed overview of the biochemical, biophysical, and protein engineering properties of glycoside hydrolases, a class of enzymes in growing use across various applications. Here, more than a dozen global experts discuss the structural and catalytic mechanisms of specific glycoside hydrolases, followed by their implications in biotechnological applications of different industrial sectors such as the food and feed industry, paper and pulp industry, the bioenergy sector and the pharmaceutical industry. Authors consider how the application of glycoside hydrolases may boost industrial production of valued products, and the broader environmental and sustainability goals of converting agrowaste into valued products. This book helps researchers and students across industry and academia gain deep knowledge of glycoside hydrolases, to advance new experimental research and applications from biofuel to drug discovery.

Details glycoside hydrolase classification, enzyme assays for biochemical characterization, and biophysical methods for structure determination and catalytic mechanisms Discusses the use of glycoside hydrolases across various applications from biofuels to drug development, enzyme technology, and fermented food production Features chapter contributions from international leaders in the field

A volume in the series on Foundations and Frontiers of Enzymology, Enzymes Beyond Traditional Applications in Dairy Science and Technology presents the applications of enzymes in dairy science and technology. Broken into four sections, this book provides a brief account of traditional applications of indigenous milk enzymes, the actions of exogenous enzymes on milk proteins for generating bioactive peptides and lactose for value addition, and methods and approaches for ensuring milk quality or cleaning milk plants.?

This book is an excellent resource for postgraduate students, academics, food scientists, and dairy professionals engaged in milk processing.

Provides coverage on in-vitro generation and in-silico prediction of bioactive peptides by action of proteolytic enzymes on major milk proteins and peptides generated during fermentation and cheese manufacturing, and their downstream processing Presents the enzymatic conversion of lactose into ?-galactooligosaccharides, or tagatose?or producing low lactose milk Discusses indigenous enzymes in normal and mastitis milk Covers regulatory policies on enzyme use in food science

Effect of High-Pressure Technologies on Enzyme: Science and Applications provides a deep, practical discussion of high-pressure processing (HPP) and high-pressure homogenization (HPH) technologies and biochemical approaches, applied across research and industry, with applications� ranging from food to pharmaceuticals and commercial enzyme production. Early chapters discuss foundational aspects of HPP and HPH approaches; the science of enzyme modification; and basic aspects of enzyme activity, stability, and structure as studied in biochemical processes. Later chapters consider the effect of HPP and HPH technologies and their mechanisms of controlling enzyme modification to improve enzyme performance for chosen applications. Special attention is paid to the application of HPP and HPH technologies and enzyme modifications in food processing, microbial enzyme modification, drug discovery, and production of other commercial enzymes, as well as the challenges of undesirable enzyme inactivation. The final chapter discusses future directions of the field and technologies, and expanded applications.

Offers a broad overview of HPP and HPH approaches and technologies applied in enzyme modification Introduces fundamental aspects of enzyme activity, stability, and structure as studied in biochemical processes and applications Discusses applications of HPP- and HPH-based enzyme modifications in food processing, microbial enzyme modification, drug discovery, and production of other commercial enzymes Includes chapter contributions from international leaders in the field, across research and industry

Structure and Intrinsic Disorder in Enzymology offers a direct, yet comprehensive presentation of the fundamental concepts, characteristics and functions of intrinsically disordered enzymes, along with valuable notes and technical insights powering new research in this emerging field. Here, more than twenty international experts examine protein flexibility and cryo-enzymology, hierarchies of intrinsic disorder, methods for measurement of disorder in proteins, bioinformatics tools for predictions of structure, disorder and function, protein promiscuity, protein moonlighting, globular enzymes, intrinsic disorder and allosteric regulation, protein crowding, intrinsic disorder in post-translational, and much more.

Chapters also review methods for study, as well as evolving technology to support new research across academic, industrial and pharmaceutical labs.

Unifies the roles of intrinsic disorder and structure in the functioning of enzymes and proteins Examines a range of enzyme and protein characteristics, their relationship to intrinsic disorder, and methods for study Features chapter contributions from international leaders in the field

The transformation of polysaccharides into valuable compounds for health and industry requires the careful application of enzyme protocols and controlled biocatalysis.

Polysaccharide-Degrading Biocatalysts provides a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels, and food engineering. Here, over a dozen leading experts offer a close examination of structural polysaccharides, genetic modification of polysaccharides, polysaccharide degradation routes, pretreatments for enzymatic hydrolysis, hemicellulose-degrading enzymes, biomass valorization processes, oligosaccharide production, and enzyme immobilization for the hydrolysis of polysaccharides, among other topics and related research protocols. A final chapter considers perspectives and challenges in an evolving, carbohydrate-based economy.

Describes the role of enzymes in the degradation of polysaccharides to obtain building blocks for biochemical processes Covers new tools for enzymatic evolution, research protocols, and process strategies contributing to large-scale applications Explores the use of polysaccharide hydrolysis products in the areas of pharmaceuticals, fuels, and food engineering Features chapter contributions from international experts

Laccase and Polyphenol Oxidase: Biochemistry and Biotechnological Applications, a new volume in the Foundations and Frontiers in Enzymology series, provides a thorough discussion of the laccase and polyphenol oxidase enzyme families, which play key roles in the oxidation of phenolic substrates and are involved in a range of important biological functions in many organisms. In recent years, significant advances have been made in understanding these enzymes, but few resources exist on their fundamental biology, mechanisms, and applications. In this book, leading world experts discuss the biochemistry of laccase and polyphenol oxidases, research methods, and uses across industries, offering mechanistic and practical overviews of these important enzyme classes and empowering researchers to develop their own studies and hone applications. This book deftly connects topics across these intricate enzyme systems, with early chapters on the physicochemical properties of PPO and laccase, followed by separate sections on these related enzyme classes’ function in biological and industrial systems, production, purification, active assays, and application areas from food and wine to bioremediation to green chemistry.. Examines the fundamental biology of laccase and polyphenol oxidases, research methods, and their uses across industries. Features chapter contributions from leading international experts in the field

Lanthanides in Enzymology and Microbiology, a new volume in the Foundations and Frontiers in Enzymology Series, offers a detailed discussion of lanthanides and lanthanide-dependent enzyme biology. In this book, more than a dozen global experts consider lanthanide enzymology fundamentals, organismal utilization of lanthanides, distribution and diversity of lanthanide-dependent enzymes, regulation of intracellular levels of lanthanides, gene expression regulation via lanthanides, as well as likely applications of lanthanide binding proteins. Lanthanide-dependent methanol and alcohol dehydrogenase metabolism are considered in both methylotrophs and non-methylotrophs, alongside various application areas, from recovery of rare earth elements to developing lanthanide ion binding peptides and biosynthesis of terpolymers through reverse-oxidation pathways. In providing this deep context and pathways for future research, this book acts as an invaluable resource in this emerging field for researchers and students of biochemistry, biotechnology, and environmental microbiology alike.Examines newly discovered biological functions of lanthanides and lanthanide dependent enzymesExplores expanding roles of lanthanides in methylotrophic and non-methylotrophic bacteriaDiscusses complex regulation mechanisms of bacterial physiology utilizing lanthanides, as well as possible application areasFeatures chapter contributions from leading, global experts in the field

Lipases: Foundations, Applications, and Perspectives, a new volume in the Foundations and Frontiers of Enzymology series, offers a practical, in-depth discussion of lipases and instruction in their use across research and industry. Here, global experts in the field introduce readers to basic lipase biology and the history of the field, interfacial activation and lipase lids, evolving analytical techniques and technology for studying lipases, and a range of lipase applications, from health and drug discovery to biofuels, food, cosmetics, and waste treatment. Lipase sources and production methods discussed include mammalian lipases, microbial lipase production, biomass lipase production, immobilized lipases, and tailor-made recombinant lipases, among other approaches.Provides an in-depth, practical discussion of lipases and current perspectives in the fieldOffers applied instruction in methods and analytical techniques for studying and using lipases across research and industryExplores a range of lipase applications, from health and drug discovery to biofuels, food, cosmetics, and waste treatmentFeatures contributions from international experts in the field

Cryptic Enzymes and Moonlighting Proteins, a new volume in the Foundations and Frontiers in Enzymology series, offers a thorough overview of cryptic enzymes and moonlighting proteins in signaling cascades. In early chapters, leading international contributors discuss evolutionary considerations for moonlighting proteins, moonlighting interactions in the extracellular matrix, eukaryotic moonlighting proteins, modulating, moonlighting kinases, moonlighting proteins in neurobiology signaling, metabolic enzymes moonlighting as RNA binding and regulatory proteins. Later, methods-driven chapters discuss practical aspects of identifying hidden moonlighting domains in proteins, computational approaches and bioinformatic tools for the identification of cryptic enzymes, establishing cryptic enzyme interactomes, and assessing contributions of moonlighting proteins to signal cascades.The book also explores recent advances in research and brings together an array of information across different fields to enable better targeting of these exciting proteins and their interactomes. With a clear focus on the role of moonlighting and cryptic enzymes in signal transduction, users will find examples of cryptic enzymes across species, as well as those in human healthy biology and pathogenesis.Covers recent advances in our understanding of cryptic moonlighting proteins in signal cascades, highlighting and examining key themes across disciplinesEmpowers researchers to better target cryptic enzymes and moonlighting proteins and their interactomes.Features chapter contributions from international leaders in the field

Sulfurtransferases: Essential Enzymes for Life stands as the first comprehensive resource on this increasingly important class of enzymes. Following an introduction to the field from the Editors, each chapter covers a specific sulfurtransferase, including its basic biology and roles in healthy functioning, disease, drug discovery, and other biotechnological applications. The physiological function for each enzyme is considered in depth, along with regulation mechanisms, pharmacological inhibitors, and pathology and conditions related to altered enzymatic activity. Sulfurtransferases discussed include rhodanese, MST, thiosulfate-thiol sulfurtransferase, tRNA uracil 4-sulfurtransferase, thiosulfate-dithiol sulfurtransferases, biotin synthase, cysteine desulfurase, lipoyl synthase, molybdenum cofactor sulfurtransferase, thiazole synthase, molybdopterin synthase sulfurtransferase, molybdopterin synthase, tRNA-uridine 2-sulfurtransferase, tRNA-5-taurinomethyluridine 2-sulfurtransferase, tRNA-5-methyluridine (54) 2-sulfurtransferase, and L-aspartate semialdehyde sulfurtransferase, among others. Here, researchers will discover key knowledge and recent advances to bring forward new studies on this increasingly relevant class of enzymes, while clinicians may apply new findings in medical practice.

Brings together basic knowledge and applied research across a wide range of sulfurtransferases Considers biochemical functioning, enzymatic regulation and activity, and related pathologies for each enzyme Features chapter contributions from global leaders in the field

Biocatalysis in Asymmetric Synthesis, a new volume in the Foundations and Frontiers of Enzymology series, offers an applied discussion on synthesizing biological catalysts using asymmetric synthesis for varying applications. Here, global experts in the field analyze a wide variety of biocatalysts and their physical states, process conditions for their asymmetric synthesis, solvents required during synthesis, and even downstream procedures for the recovery of final products. The book adopts an interdisciplinary approach, merging fundamental biology and its synthetic applications across industries with a wide range of practical examples, from directed evolution to biotransformation and production of novel enzymes and non-conventional catalysts. Throughout the book, the impact and application of biocatalysis in sustainable processing is considered in-depth. This book will also help non-experts in biocatalysis to apply this knowledge in their own research, providing a thorough overview of the ways asymmetric biocatalytic approaches may be adapted for different disciplines and downstream products.Explores biocatalysts as exquisite catalysts for fine chiral compound synthesis in different reaction mediaFeatures both foundational overviews and applied, practical examples across research and industryIncludes chapter contributions from international leaders in the field

Sustainable Process Intensification for Biocatalysis: Applications in Enzymology and Biotechnology, a new volume in the Foundations and Frontiers of Enzymology series, offers applied instruction in process intensification principles for applications in enzymology and biotechnology, helping researchers and practitioners implement these techniques across various uses and industries, from healthcare to food and biofuel. Leaders in the field discuss a full range of approaches and enabling technologies, from microwave to ultrasound, hydrodynamic cavitation, and combination chemical and biological catalysis techniques, such as multiphase reactors, microreactors, scale-bioprocesses, and enzyme cascades.Later chapters examine ongoing advances and their implementation across different industries, including pharmaceuticals, perfumes, polymers, and agrochemicals, with a final chapter discussing next steps in research and future directions. Throughout the book, and across applied examples, emphasis is placed on driving sustainable research approaches and manufacturing.Supports researchers and practitioners in improving the activity, stability, and reusability of enzymes across various research disciplines and industriesConsiders a wide range of process intensification techniques, including microwave, ultrasound, hydrodynamic cavitation, and combination chemical and biological catalysis approachesFeatures chapter contributions from international leaders in the field

Transglutaminase: Fundamentals and Applications, a new volume in the Foundations and Frontiers in Enzymology series, stands as a comprehensive resource on this increasingly important class of enzymes. Following an introduction to the field, international experts discuss basic biology and structure - function relationships of transglutaminases, ongoing fundamental research, and new application areas, including food processing, material synthesis, biomedicine, disease treatment, and biotechnology. Approaches for sourcing transglutaminases from animals, plants, and microorganisms are considered, followed by chapters on applied uses in biomedicine, skin, bone biology, medicine, drug development, tissue engineering, seafood bioprocessing, dairy bioprocessing, synthesis of materials and polymers, and as an immobilization agent.Research protocols are also provided, including research rationale, design, substrates, assays, and microassay steps for studying transglutaminases.Brings together basic knowledge and applied research on transglutaminase biologyConsiders a range of application areas, from biomedicine to drug development, tissue engineering, food processing, and materials synthesisIncludes instructive protocols for basic and applied transglutaminase researchFeatures chapter contributions from global leading experts in the field, across disciplines and applications

Isothermal Titration Calorimetry in Enzymology: Techniques and Applications provides a thorough, practical overview of ITC as a productive and powerful tool for quantifying enzyme catalysis and interactions with substrates, products, cofactors, and inhibitors. Here, global experts in the methodology discuss the full capabilities of ITC and how it may be applied in research, medicine, and industry and offer a basis to continue leveraging ITC as its uses expand and evolve. This book bridges the gap between research and application by giving extensive technical details on cutting edge ITC techniques across a wide swath of both ITC kinetics measurements and ITC thermodynamic measurements, alongside case studies illustrating the depth of knowledge that can be obtained. Applications discussed include measuring binding kinetics by ITC, analyzing ITC kinetic data, in vivo enzyme kinetics by ITC, analyzing enzyme kinetics in crowded solutions, studying catalysis of biological substrates, interpreting ITC binding curves, ITC in drug discovery, ITC in the development of cancer therapeutics, ITC analysis of aminoglycoside resistance enzymes, studying enzyme interactions with toxic metals, using ITC to study ubiquitination, and ITC studies of glycoside hydrolases, among others.Describes and instructs in cutting-edge ITC experiments and analysis techniquesCovers both kinetics and thermodynamics-based ITC approachesFeatures illustrative case studies across a wide range of applicationsIncludes chapter contributions from international experts in ITC

Bacterial Enzymes as Targets for Drug Discovery: Meeting the Challenges of Antibiotic Resistance addresses the gap between medical microbiology, structural biology, and genomic science in the development of new antibacterial drug development. This book consolidates detailed profiling of bacterial target enzyme families for the drug discovery process and methodologies for use and validation of the potential drug targets. The contents cover the foundations of the antibiotic drug discovery process and focus on bacterial enzymes as drug targets, building across these disciplines to provide a comprehensive resource in bacterial structural biology and genomics. This is the ideal reference for antibiotic drug discovery researchers in the pharma industry and academia. Biochemists, microbiologists, and medicinal chemists will also benefit from this books’ content.

Provides strategies and approaches to drug design aiming at overcoming antibiotic resistance.Includes most common roadblocks in identifying novel drug targets and presents the strategies to overcome.Provides potential methods to identify new drug targets by genome mining.

Cold-Adapted Enzymes: Biochemistry and Biotechnology provides a cutting-edge investigation into the molecular mechanisms, structural dynamics, and regulatory pathways of enzymes adapted to cold environments.Beyond classical microbiology, the book explores cold adaptation from psychrophilic bacteria and Antarctic fungi to microalgae, plants, and mammalian cell physiology. It provides biochemistry insights into carbohydrate (amylases, glucoamylases) and lipid metabolism, proteases, and redox enzymes, alongside the roles of molecular chaperones and prokaryotic proteomics. A key highlight is its focus on next-generation biotechnology, featuring AI-driven protein design, advanced computational modeling, and structural bioinformatics. Bridging foundational science with market reality, the volume explores applications from organic waste valorization to industrial biocatalysis, analyzing the global patent landscape and commercial players.As the latest volume in the Foundations and Frontiers of Enzymology series, this book is an indispensable reference for researchers across biochemistry, biotechnology, structural biology, and microbiology.

Covers the molecular mechanisms developed by enzymes to improve their stability and compensate for the effects of cold exposureStudies the structure and biochemical properties of cold-adapted enzymesExplores current and potential applications of cold-adapted enzymes in different types of industries

Enzymatic Production of Oligosaccharides: From Basic Research to Industrial Production, a volume in the Foundations and Frontiers of Enzymology series, covers the enzymes used for the extraction, synthesis, or modification of oligosaccharides, the biochemical properties of those enzymes, and potential technological applications. The book is divided into three parts, the first of which shares the fundamentals on the enzymatic synthesis of oligosaccharides. The book then explores the enzymatic production of oligosaccharides, considering a range of different oligosaccharides and production processes. Final sections cover the technological properties of oligosaccharides and potential applications across the chemical, biochemical, biotechnology, and food industries.This is a useful reference for researchers working with oligosaccharides across biochemistry, enzymology, biotechnology, food chemistry and related fields.

Focuses on a range of different oligosaccharides, including chito-oligosaccharides, xylo-oligosaccharides, galacto-oligosaccharides and lactulose-derived oligosaccharidesIncludes various production processes of oligosaccharides, such as enzymatic production and microbial productionAnalyzes enzyme reactors used for oligosaccharides synthesisDiscusses the downstream processing of oligosaccharides for product separation and purificationConsiders food, cosmetic, and pharmaceutic applications of oligosaccharides

Whole Cell Biocatalysis, a volume in the Foundations and Frontiers of Enzymology series, offers a detailed overview of the process of biocatalysis using whole cells as an alternative to enzyme biocatalysis. The book examines the potential applications and advantages of whole cell biocatalysis, including its use in the production of fine chemicals, renewable energy, and drug discovery and development. Whole cell biocatalysis for large scale production and non-conventional media are also covered. In addition, the latest methods and techniques are investigated, including cell immobilization, permeabilization, synthetic biology, computational metabolic engineering, and molecular genetics.This book provides a comprehensive summary on whole cell biocatalysis and the latest developments in this emerging field. It is an invaluable reference for researchers working across biochemistry, enzymology, biotechnology, and related fields.

Considers the advantages and challenges of whole cell biocatalysis to enhance production processes in various industriesIncludes a range of techniques to investigate and facilitate whole cell biocatalysisCovers whole cell biocatalysis in non-conventional mediaStudies biocatalytic cascade reactions

RNases in Health and Diseases: From Housekeeping Enzymes to Moonlight Proteins provides coverage of the structural and functional properties of RNases, introducing new concepts such as their recently discovered multifaceted properties, their involvement in the generation of non-coding signaling RNAs, and their proposed uses as diagnostic and therapeutic tools. This book's sections cover the structure of RNases, present research methods and tools to study RNases, and delve into RNases and diseases. Users will find a wider perspective on the topics discussed that will serve researchers and students engaged in the study of RNases who are interested in acquiring a better understanding.

Covers basic theoretical information, methodological tools, and potential therapeutic applications in the RNase fieldIntroduces new concepts to the RNase fieldIncludes both the historical background in the field and the most recent advancesContains contributions from international experts in the field

Navigating Tuberculosis: Insights into Host-Pathogen Protein Interactions, the latest release in the Foundations and Frontiers in Enzymology series, explores the interactions between host proteins and the tuberculosis pathogen, Mycobacterium tuberculosis (M. tb), emphasizing their roles in virulence and pathogenesis. The book delves into mechanistic insights, focusing on their roles in modulating innate and adaptive immunity and understanding TB pathomechanisms by elucidating the interplay between host defenses and M. tb infection. It provides a foundation for developing targeted mitigation strategies and discusses key regulatory pathways and protein factors involved in host-pathogen interactions, informing the design and implementation of innovative approaches to combat TB.This comprehensive volume aims to advance knowledge and strategies to combat tuberculosis, aligning with the World Health Organization's goal of eradicating TB by 2035 by addressing the need for up-to-date information on host-pathogen interactions in tuberculosis and incorporating the latest research findings and advancements. It highlights emerging therapeutic targets among host and TB pathogen proteins and integrates multidisciplinary approaches from microbiology, immunology, and molecular biology, offering a holistic perspective on TB pathophysiology. Researchers will find detailed discussions on key host proteins involved in tuberculosis pathogenesis, the molecular tactics employed by M. tb to subvert host defenses, and host-mediated inhibition strategies targeting M. tb.Provides comprehensive insights into the complex interactions between host proteins and the tuberculosis pathogen, revealing essential aspects of tuberculosis pathophysiologyInvestigates the latest discoveries and breakthroughs in the field, presented by prominent authorities, to uncover innovative approaches for diagnosing and treating tuberculosis, including novel therapeutic targetsOffers a multidisciplinary perspective that combines insights from microbiology, immunology, biochemistry, and molecular biology, fostering a comprehensive understanding of host-pathogen interactions in tuberculosisHighlights cutting-edge research on key host and pathogen proteins involved in tuberculosis, offering detailed discussions on their roles in modulating innate and adaptive immunityExplores host-mediated inhibition strategies targeting Mycobacterium tuberculosis, paving the way for the development of novel therapeutic interventions to bolster host defenses against TB

Protein Dynamics: Experimental and Computational Approaches investigates protein biology from a biophysical standpoint. The book covers a wide range of topics, including functional protein dynamics, intrinsically disordered proteins, protein folding, misfolding and aggregation, and membrane protein-protein communication. Protein allosteric conformational changes caused by drug binding and disease-associated mutations are also featured, alongside protein dynamics in the context of computer-based drug design. Biophysical techniques for investigating protein structure-dynamics-function relationships across these topics are explored, considering the latest experimental and computational approaches in the field. This book is an ideal reference for researchers in working in biophysics, biochemistry, and related areas.Covers a broad range of biophysical techniques to study protein dynamicsInvestigates an array of different proteins, including receptors, transporters and enzymesConsiders future directions in the fieldFeatures explanation of key concepts and end-of-chapter questions to aid understanding

Enzyme Kinetics: Fundamentals, Applications, and Reactor Design is a comprehensive guide that provides readers with a solid grounding in the principles of enzyme kinetics and bioreactor design while also illuminating their practical applications. The book takes a step-by-step approach, starting from basic concepts and gradually moving to advanced topics, to ensure that readers develop a strong understanding and are well-equipped to apply this knowledge in real-world scenarios. “Enzyme Kinetics: Fundamentals, Applications, and Reactor Design” covers crucial areas including safety regulations and future trends, making it an all-in-one resource for anyone interested in these fields. It also provides foundational information, explains methods and computational approaches, and presents case studies and applications. This book is designed to serve as a valuable resource for a broad range of readers, including undergraduate and graduate students studying biochemistry, biotechnology, and chemical engineering, as well as professionals and researchers looking to deepen their knowledge and stay at the forefront of their field. Enzyme Kinetics: Fundamentals, Applications, and Reactor Design offers an integrative and up-to-date perspective on enzyme kinetics and bioreactor design, with an emphasis on real-world applications, cutting-edge developments, and safety regulations.Offers a complete and up-to-date overview of enzyme kinetics and bioreactor design, moving from fundamental concepts to advanced topics and covering crucial areas like safety regulations and future trends, making it an all-in-one resource for anyone interested in these fieldsUniquely bridges the gap between theory and practice, with real-world applications and case studies integrated throughout the content, enabling readers to directly apply what they learn to their work or researchPresents information in a clear and accessible manner, with concepts broken down step-by-step to ensure they are easy to grasp, making this book an invaluable tool for both beginners and experienced professionals alike

Carbonyl Reductase: Design, Screening, Modification and Industrial Applications promotes the practical applications of carbonyl reductase, introducing the most recent advances of carbonyl reductases, including the analyzed crystal structures and structural information, the catalytic mechanism, the high-throughput screening methods, the protein engineering and the expanded applications. It is a valuable resource for researchers and professionals interested in carbonyl reductase and chiral γ-amino alcohols production.

Super-Resolution Nanoscopy for Protein Imaging decodes the intricate world of protein visualization using cutting-edge nanoscopic techniques. This comprehensive guide is crafted to equip master students, PhD candidates, and early-stage researchers with essential knowledge and practical insights into advanced protein imaging technologies. The book covers foundational principles, innovative methodologies, and real-world applications, providing a complete resource for mastering the complexities of protein imaging. This book offers a unique compilation of diverse nanoscopic methods, overcoming the limitation of technique-specific texts currently available.By collecting scattered information into a single volume, it delivers an in-depth exploration of imaging techniques, from Atomic Force Microscopy (AFM) to Electron Microscopy (EM), and Single Molecule Fluorescence Imaging. Each section delves into instrumentation, sample preparation, data analysis, and the latest developments, making it an indispensable reference for understanding how to visualize proteins in their natural environments.Offers a comprehensive guide to various super-resolution nanoscopy techniques, providing in-depth knowledge on methodologies for imaging different types of proteinsExplains advanced instrumentation and sample preparation methods, ensuring readers can effectively utilize high-resolution imaging toolsDetails the strengths and limitations of various imaging techniques, helping researchers choose the most appropriate method for their specific needsShowcases real-world applications and case studies, demonstrating how these imaging techniques can be applied in biological research and future developmentsProvides insights into cutting-edge developments and future directions in protein imaging, empowering researchers to stay ahead in this rapidly evolving field

Biocatalysis in Green Solvents offers a pragmatic overview and instruction in biocatalysis and enzymology of green solvents for sustainable industries and medicine, running from concept to application. Here, international experts in the field discuss structure-function relationships of enzymes in ionic liquids (ILs) and examine how enzymes act as selective catalysts for fine biochemical synthesis in non-aqueous environments. Several integral green biochemical processes of biocatalytic transformation and pure product separation are described in detail.

Application focused chapters discuss the role of biocatalysis in creating and implementing deep eutectic solvents, biomass derived solvents, sub and supercritical fluids, carbon dioxide biphasic systems, and enzymatic membrane reactors, as well as applying these biocatalytic processes in drug discovery and production.

Examines the structure-function relationships of enzymes in ionic liquids and biocatalytic processes for various applications across industry and medicine Includes clear instruction in core green biochemical processes-of (bio)catalytic transformation-and pure product separation Features chapter contributions from international experts across academia and industry

Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes.

Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.

Provides a thorough overview of biocatalyst and enzyme immobilization for research and practical application Presents methods based content that instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization Features chapter contributions from international leaders in the field

Biosurfactants: Research and Development provides a thorough overview of biosurfactant research and development across a range of settings and industries, highlighting the novel use of enzymes, metabolic and genetic engineering in biosurfactant production and showcasing diverse experimental models and approaches. Sections discuss fundamental characteristics of biosurfactants, their physio-chemical properties, and their differences from chemically synthesize surfactants, different research approaches for the study of known biosurfactants, and the genetic manipulation of microorganisms to increase biosurfactant productivity, or to produce molecules with improved characteristics.

Throughout the book, methods and approaches are discussed in easy-to-digest formats, with methods discussed, ranging from in silico approaches to classical biocatalysis omics analysis and metabolic engineering.

Provides a through overview of biosurfactant enzymes and microorganisms used in biosurfactant production Features instruction in a wide range of research and development approaches, ranging from in silico techniques to classical biocatalysis omics analysis and metabolic engineering Features chapter contributions from international leaders in the field

Ureases: Foundations, Classes, and Applications provides a thorough, practical analysis of ureases-enzymes of growing relevance across a range of biotechnological applications and drug discovery. Unique in many aspects, ureases are one of the few enzymes to have nickel in their active sites. This book covers all aspects of this enzymatic class starting with foundational overview and then providing historical urease research and current state, from basic biochemistry to the use of ureases as hallmarks in enzymology, crystallography, and bioinorganic chemistry. The different classes of ureases, structurally diverse but chemically equivalent, are individually discussed.The multi-protein, multi-step activation of ureases (with chemical modification of residues, transport, and transfer of nickel ions) are examined in-depth, along with the catalytic mechanisms of ureolysis and its inhibitors. The final two sections of the book address multiple applications of ureases in health and biotechnology, respectively, going from gastric ulcer treatment to architectural uses in buildings and engineering. Future applications and next steps in research are also considered.

Considers fundamental aspects of urease biochemistry, ureolysis and urease inhibitorsDiscusses urease research across a range of applications, from drug discovery to biosensors, water purification, architecture and the food industryFeatures chapter contributions from international leaders in the field

Proteolytic enzymes are found in all life forms, where they catalyze peptide bond hydrolysis during essential biological processes. Proteases of various pathogens have also been shown to use endogenous protease inhibitors to regulate the activity of host proteolytic enzymes encountered during infection.Proteolytic Enzymes and their Inhibitors in Infectious Pathogens brings together key experts to provide a thorough discussion of proteolytic enzymes and their endogenous inhibitors found in parasites, bacteria, and viruses. The book focuses on the respective roles of the different catalytic classes of proteolytic enzymes and their protein-protease inhibitors at the host-infectious organism interface, and how this knowledge may inform drug and vaccine design. Following a foundational overview of proteases and macromolecular inhibitors of pathogenic organisms, authors discuss recent research on distinct classes of proteolytic enzymes in pathogens. Classes include aspartic proteases, cysteine proteases, metalloproteases, serine proteases, and threonine proteases with experts contributing chapters on distinct enzymes and inhibitors. New methods to identify protease substrates and inhibitors are also provided, with step-by-step protocols to advance new research and drive drug discovery.Discusses proteolytic enzymes of parasites, bacteria, and viruses arranged by peptide bond cleavage classes, for improved integration of information across the infectious organismsIncludes information on the structure and interaction with proteases of macromolecular protease inhibitors, and how these structures can be engineered to provide more potent and specific protease inhibitorsProvides instructions in methods and technologies to identify physiological protease substrates, their cleavage specificities, and inhibitorsFeatures rich illustrations and diagrams of core concepts, including informative three-dimensional protein structures and molecular interactions

Glycoside Hydrolases provides a detailed overview of the biochemical, biophysical, and protein engineering properties of glycoside hydrolases, a class of enzymes in growing use across various applications. Here, more than a dozen global experts discuss the structural and catalytic mechanisms of specific glycoside hydrolases, followed by their implications in biotechnological applications of different industrial sectors such as the food and feed industry, paper and pulp industry, the bioenergy sector and the pharmaceutical industry. Authors consider how the application of glycoside hydrolases may boost industrial production of valued products, and the broader environmental and sustainability goals of converting agrowaste into valued products. This book helps researchers and students across industry and academia gain deep knowledge of glycoside hydrolases, to advance new experimental research and applications from biofuel to drug discovery.

Details glycoside hydrolase classification, enzyme assays for biochemical characterization, and biophysical methods for structure determination and catalytic mechanisms Discusses the use of glycoside hydrolases across various applications from biofuels to drug development, enzyme technology, and fermented food production Features chapter contributions from international leaders in the field

A volume in the series on Foundations and Frontiers of Enzymology, Enzymes Beyond Traditional Applications in Dairy Science and Technology presents the applications of enzymes in dairy science and technology. Broken into four sections, this book provides a brief account of traditional applications of indigenous milk enzymes, the actions of exogenous enzymes on milk proteins for generating bioactive peptides and lactose for value addition, and methods and approaches for ensuring milk quality or cleaning milk plants.?

This book is an excellent resource for postgraduate students, academics, food scientists, and dairy professionals engaged in milk processing.

Provides coverage on in-vitro generation and in-silico prediction of bioactive peptides by action of proteolytic enzymes on major milk proteins and peptides generated during fermentation and cheese manufacturing, and their downstream processing Presents the enzymatic conversion of lactose into ?-galactooligosaccharides, or tagatose?or producing low lactose milk Discusses indigenous enzymes in normal and mastitis milk Covers regulatory policies on enzyme use in food science

Effect of High-Pressure Technologies on Enzyme: Science and Applications provides a deep, practical discussion of high-pressure processing (HPP) and high-pressure homogenization (HPH) technologies and biochemical approaches, applied across research and industry, with applications� ranging from food to pharmaceuticals and commercial enzyme production. Early chapters discuss foundational aspects of HPP and HPH approaches; the science of enzyme modification; and basic aspects of enzyme activity, stability, and structure as studied in biochemical processes. Later chapters consider the effect of HPP and HPH technologies and their mechanisms of controlling enzyme modification to improve enzyme performance for chosen applications. Special attention is paid to the application of HPP and HPH technologies and enzyme modifications in food processing, microbial enzyme modification, drug discovery, and production of other commercial enzymes, as well as the challenges of undesirable enzyme inactivation. The final chapter discusses future directions of the field and technologies, and expanded applications.

Offers a broad overview of HPP and HPH approaches and technologies applied in enzyme modification Introduces fundamental aspects of enzyme activity, stability, and structure as studied in biochemical processes and applications Discusses applications of HPP- and HPH-based enzyme modifications in food processing, microbial enzyme modification, drug discovery, and production of other commercial enzymes Includes chapter contributions from international leaders in the field, across research and industry

Structure and Intrinsic Disorder in Enzymology offers a direct, yet comprehensive presentation of the fundamental concepts, characteristics and functions of intrinsically disordered enzymes, along with valuable notes and technical insights powering new research in this emerging field. Here, more than twenty international experts examine protein flexibility and cryo-enzymology, hierarchies of intrinsic disorder, methods for measurement of disorder in proteins, bioinformatics tools for predictions of structure, disorder and function, protein promiscuity, protein moonlighting, globular enzymes, intrinsic disorder and allosteric regulation, protein crowding, intrinsic disorder in post-translational, and much more.

Chapters also review methods for study, as well as evolving technology to support new research across academic, industrial and pharmaceutical labs.

Unifies the roles of intrinsic disorder and structure in the functioning of enzymes and proteins Examines a range of enzyme and protein characteristics, their relationship to intrinsic disorder, and methods for study Features chapter contributions from international leaders in the field

The transformation of polysaccharides into valuable compounds for health and industry requires the careful application of enzyme protocols and controlled biocatalysis.

Polysaccharide-Degrading Biocatalysts provides a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels, and food engineering. Here, over a dozen leading experts offer a close examination of structural polysaccharides, genetic modification of polysaccharides, polysaccharide degradation routes, pretreatments for enzymatic hydrolysis, hemicellulose-degrading enzymes, biomass valorization processes, oligosaccharide production, and enzyme immobilization for the hydrolysis of polysaccharides, among other topics and related research protocols. A final chapter considers perspectives and challenges in an evolving, carbohydrate-based economy.

Describes the role of enzymes in the degradation of polysaccharides to obtain building blocks for biochemical processes Covers new tools for enzymatic evolution, research protocols, and process strategies contributing to large-scale applications Explores the use of polysaccharide hydrolysis products in the areas of pharmaceuticals, fuels, and food engineering Features chapter contributions from international experts

Laccase and Polyphenol Oxidase: Biochemistry and Biotechnological Applications, a new volume in the Foundations and Frontiers in Enzymology series, provides a thorough discussion of the laccase and polyphenol oxidase enzyme families, which play key roles in the oxidation of phenolic substrates and are involved in a range of important biological functions in many organisms. In recent years, significant advances have been made in understanding these enzymes, but few resources exist on their fundamental biology, mechanisms, and applications. In this book, leading world experts discuss the biochemistry of laccase and polyphenol oxidases, research methods, and uses across industries, offering mechanistic and practical overviews of these important enzyme classes and empowering researchers to develop their own studies and hone applications. This book deftly connects topics across these intricate enzyme systems, with early chapters on the physicochemical properties of PPO and laccase, followed by separate sections on these related enzyme classes’ function in biological and industrial systems, production, purification, active assays, and application areas from food and wine to bioremediation to green chemistry.. Examines the fundamental biology of laccase and polyphenol oxidases, research methods, and their uses across industries. Features chapter contributions from leading international experts in the field

Lanthanides in Enzymology and Microbiology, a new volume in the Foundations and Frontiers in Enzymology Series, offers a detailed discussion of lanthanides and lanthanide-dependent enzyme biology. In this book, more than a dozen global experts consider lanthanide enzymology fundamentals, organismal utilization of lanthanides, distribution and diversity of lanthanide-dependent enzymes, regulation of intracellular levels of lanthanides, gene expression regulation via lanthanides, as well as likely applications of lanthanide binding proteins. Lanthanide-dependent methanol and alcohol dehydrogenase metabolism are considered in both methylotrophs and non-methylotrophs, alongside various application areas, from recovery of rare earth elements to developing lanthanide ion binding peptides and biosynthesis of terpolymers through reverse-oxidation pathways. In providing this deep context and pathways for future research, this book acts as an invaluable resource in this emerging field for researchers and students of biochemistry, biotechnology, and environmental microbiology alike.Examines newly discovered biological functions of lanthanides and lanthanide dependent enzymesExplores expanding roles of lanthanides in methylotrophic and non-methylotrophic bacteriaDiscusses complex regulation mechanisms of bacterial physiology utilizing lanthanides, as well as possible application areasFeatures chapter contributions from leading, global experts in the field

Lipases: Foundations, Applications, and Perspectives, a new volume in the Foundations and Frontiers of Enzymology series, offers a practical, in-depth discussion of lipases and instruction in their use across research and industry. Here, global experts in the field introduce readers to basic lipase biology and the history of the field, interfacial activation and lipase lids, evolving analytical techniques and technology for studying lipases, and a range of lipase applications, from health and drug discovery to biofuels, food, cosmetics, and waste treatment. Lipase sources and production methods discussed include mammalian lipases, microbial lipase production, biomass lipase production, immobilized lipases, and tailor-made recombinant lipases, among other approaches.Provides an in-depth, practical discussion of lipases and current perspectives in the fieldOffers applied instruction in methods and analytical techniques for studying and using lipases across research and industryExplores a range of lipase applications, from health and drug discovery to biofuels, food, cosmetics, and waste treatmentFeatures contributions from international experts in the field

Cryptic Enzymes and Moonlighting Proteins, a new volume in the Foundations and Frontiers in Enzymology series, offers a thorough overview of cryptic enzymes and moonlighting proteins in signaling cascades. In early chapters, leading international contributors discuss evolutionary considerations for moonlighting proteins, moonlighting interactions in the extracellular matrix, eukaryotic moonlighting proteins, modulating, moonlighting kinases, moonlighting proteins in neurobiology signaling, metabolic enzymes moonlighting as RNA binding and regulatory proteins. Later, methods-driven chapters discuss practical aspects of identifying hidden moonlighting domains in proteins, computational approaches and bioinformatic tools for the identification of cryptic enzymes, establishing cryptic enzyme interactomes, and assessing contributions of moonlighting proteins to signal cascades.The book also explores recent advances in research and brings together an array of information across different fields to enable better targeting of these exciting proteins and their interactomes. With a clear focus on the role of moonlighting and cryptic enzymes in signal transduction, users will find examples of cryptic enzymes across species, as well as those in human healthy biology and pathogenesis.Covers recent advances in our understanding of cryptic moonlighting proteins in signal cascades, highlighting and examining key themes across disciplinesEmpowers researchers to better target cryptic enzymes and moonlighting proteins and their interactomes.Features chapter contributions from international leaders in the field

Sulfurtransferases: Essential Enzymes for Life stands as the first comprehensive resource on this increasingly important class of enzymes. Following an introduction to the field from the Editors, each chapter covers a specific sulfurtransferase, including its basic biology and roles in healthy functioning, disease, drug discovery, and other biotechnological applications. The physiological function for each enzyme is considered in depth, along with regulation mechanisms, pharmacological inhibitors, and pathology and conditions related to altered enzymatic activity. Sulfurtransferases discussed include rhodanese, MST, thiosulfate-thiol sulfurtransferase, tRNA uracil 4-sulfurtransferase, thiosulfate-dithiol sulfurtransferases, biotin synthase, cysteine desulfurase, lipoyl synthase, molybdenum cofactor sulfurtransferase, thiazole synthase, molybdopterin synthase sulfurtransferase, molybdopterin synthase, tRNA-uridine 2-sulfurtransferase, tRNA-5-taurinomethyluridine 2-sulfurtransferase, tRNA-5-methyluridine (54) 2-sulfurtransferase, and L-aspartate semialdehyde sulfurtransferase, among others. Here, researchers will discover key knowledge and recent advances to bring forward new studies on this increasingly relevant class of enzymes, while clinicians may apply new findings in medical practice.

Brings together basic knowledge and applied research across a wide range of sulfurtransferases Considers biochemical functioning, enzymatic regulation and activity, and related pathologies for each enzyme Features chapter contributions from global leaders in the field

Biocatalysis in Asymmetric Synthesis, a new volume in the Foundations and Frontiers of Enzymology series, offers an applied discussion on synthesizing biological catalysts using asymmetric synthesis for varying applications. Here, global experts in the field analyze a wide variety of biocatalysts and their physical states, process conditions for their asymmetric synthesis, solvents required during synthesis, and even downstream procedures for the recovery of final products. The book adopts an interdisciplinary approach, merging fundamental biology and its synthetic applications across industries with a wide range of practical examples, from directed evolution to biotransformation and production of novel enzymes and non-conventional catalysts. Throughout the book, the impact and application of biocatalysis in sustainable processing is considered in-depth. This book will also help non-experts in biocatalysis to apply this knowledge in their own research, providing a thorough overview of the ways asymmetric biocatalytic approaches may be adapted for different disciplines and downstream products.Explores biocatalysts as exquisite catalysts for fine chiral compound synthesis in different reaction mediaFeatures both foundational overviews and applied, practical examples across research and industryIncludes chapter contributions from international leaders in the field

Sustainable Process Intensification for Biocatalysis: Applications in Enzymology and Biotechnology, a new volume in the Foundations and Frontiers of Enzymology series, offers applied instruction in process intensification principles for applications in enzymology and biotechnology, helping researchers and practitioners implement these techniques across various uses and industries, from healthcare to food and biofuel. Leaders in the field discuss a full range of approaches and enabling technologies, from microwave to ultrasound, hydrodynamic cavitation, and combination chemical and biological catalysis techniques, such as multiphase reactors, microreactors, scale-bioprocesses, and enzyme cascades.Later chapters examine ongoing advances and their implementation across different industries, including pharmaceuticals, perfumes, polymers, and agrochemicals, with a final chapter discussing next steps in research and future directions. Throughout the book, and across applied examples, emphasis is placed on driving sustainable research approaches and manufacturing.Supports researchers and practitioners in improving the activity, stability, and reusability of enzymes across various research disciplines and industriesConsiders a wide range of process intensification techniques, including microwave, ultrasound, hydrodynamic cavitation, and combination chemical and biological catalysis approachesFeatures chapter contributions from international leaders in the field

Transglutaminase: Fundamentals and Applications, a new volume in the Foundations and Frontiers in Enzymology series, stands as a comprehensive resource on this increasingly important class of enzymes. Following an introduction to the field, international experts discuss basic biology and structure - function relationships of transglutaminases, ongoing fundamental research, and new application areas, including food processing, material synthesis, biomedicine, disease treatment, and biotechnology. Approaches for sourcing transglutaminases from animals, plants, and microorganisms are considered, followed by chapters on applied uses in biomedicine, skin, bone biology, medicine, drug development, tissue engineering, seafood bioprocessing, dairy bioprocessing, synthesis of materials and polymers, and as an immobilization agent.Research protocols are also provided, including research rationale, design, substrates, assays, and microassay steps for studying transglutaminases.Brings together basic knowledge and applied research on transglutaminase biologyConsiders a range of application areas, from biomedicine to drug development, tissue engineering, food processing, and materials synthesisIncludes instructive protocols for basic and applied transglutaminase researchFeatures chapter contributions from global leading experts in the field, across disciplines and applications

Isothermal Titration Calorimetry in Enzymology: Techniques and Applications provides a thorough, practical overview of ITC as a productive and powerful tool for quantifying enzyme catalysis and interactions with substrates, products, cofactors, and inhibitors. Here, global experts in the methodology discuss the full capabilities of ITC and how it may be applied in research, medicine, and industry and offer a basis to continue leveraging ITC as its uses expand and evolve. This book bridges the gap between research and application by giving extensive technical details on cutting edge ITC techniques across a wide swath of both ITC kinetics measurements and ITC thermodynamic measurements, alongside case studies illustrating the depth of knowledge that can be obtained. Applications discussed include measuring binding kinetics by ITC, analyzing ITC kinetic data, in vivo enzyme kinetics by ITC, analyzing enzyme kinetics in crowded solutions, studying catalysis of biological substrates, interpreting ITC binding curves, ITC in drug discovery, ITC in the development of cancer therapeutics, ITC analysis of aminoglycoside resistance enzymes, studying enzyme interactions with toxic metals, using ITC to study ubiquitination, and ITC studies of glycoside hydrolases, among others.Describes and instructs in cutting-edge ITC experiments and analysis techniquesCovers both kinetics and thermodynamics-based ITC approachesFeatures illustrative case studies across a wide range of applicationsIncludes chapter contributions from international experts in ITC

Bacterial Enzymes as Targets for Drug Discovery: Meeting the Challenges of Antibiotic Resistance addresses the gap between medical microbiology, structural biology, and genomic science in the development of new antibacterial drug development. This book consolidates detailed profiling of bacterial target enzyme families for the drug discovery process and methodologies for use and validation of the potential drug targets. The contents cover the foundations of the antibiotic drug discovery process and focus on bacterial enzymes as drug targets, building across these disciplines to provide a comprehensive resource in bacterial structural biology and genomics. This is the ideal reference for antibiotic drug discovery researchers in the pharma industry and academia. Biochemists, microbiologists, and medicinal chemists will also benefit from this books’ content.

Provides strategies and approaches to drug design aiming at overcoming antibiotic resistance.Includes most common roadblocks in identifying novel drug targets and presents the strategies to overcome.Provides potential methods to identify new drug targets by genome mining.

Cold-Adapted Enzymes: Biochemistry and Biotechnology provides a cutting-edge investigation into the molecular mechanisms, structural dynamics, and regulatory pathways of enzymes adapted to cold environments.Beyond classical microbiology, the book explores cold adaptation from psychrophilic bacteria and Antarctic fungi to microalgae, plants, and mammalian cell physiology. It provides biochemistry insights into carbohydrate (amylases, glucoamylases) and lipid metabolism, proteases, and redox enzymes, alongside the roles of molecular chaperones and prokaryotic proteomics. A key highlight is its focus on next-generation biotechnology, featuring AI-driven protein design, advanced computational modeling, and structural bioinformatics. Bridging foundational science with market reality, the volume explores applications from organic waste valorization to industrial biocatalysis, analyzing the global patent landscape and commercial players.As the latest volume in the Foundations and Frontiers of Enzymology series, this book is an indispensable reference for researchers across biochemistry, biotechnology, structural biology, and microbiology.

Covers the molecular mechanisms developed by enzymes to improve their stability and compensate for the effects of cold exposureStudies the structure and biochemical properties of cold-adapted enzymesExplores current and potential applications of cold-adapted enzymes in different types of industries

Enzymatic Production of Oligosaccharides: From Basic Research to Industrial Production, a volume in the Foundations and Frontiers of Enzymology series, covers the enzymes used for the extraction, synthesis, or modification of oligosaccharides, the biochemical properties of those enzymes, and potential technological applications. The book is divided into three parts, the first of which shares the fundamentals on the enzymatic synthesis of oligosaccharides. The book then explores the enzymatic production of oligosaccharides, considering a range of different oligosaccharides and production processes. Final sections cover the technological properties of oligosaccharides and potential applications across the chemical, biochemical, biotechnology, and food industries.This is a useful reference for researchers working with oligosaccharides across biochemistry, enzymology, biotechnology, food chemistry and related fields.

Focuses on a range of different oligosaccharides, including chito-oligosaccharides, xylo-oligosaccharides, galacto-oligosaccharides and lactulose-derived oligosaccharidesIncludes various production processes of oligosaccharides, such as enzymatic production and microbial productionAnalyzes enzyme reactors used for oligosaccharides synthesisDiscusses the downstream processing of oligosaccharides for product separation and purificationConsiders food, cosmetic, and pharmaceutic applications of oligosaccharides

Whole Cell Biocatalysis, a volume in the Foundations and Frontiers of Enzymology series, offers a detailed overview of the process of biocatalysis using whole cells as an alternative to enzyme biocatalysis. The book examines the potential applications and advantages of whole cell biocatalysis, including its use in the production of fine chemicals, renewable energy, and drug discovery and development. Whole cell biocatalysis for large scale production and non-conventional media are also covered. In addition, the latest methods and techniques are investigated, including cell immobilization, permeabilization, synthetic biology, computational metabolic engineering, and molecular genetics.This book provides a comprehensive summary on whole cell biocatalysis and the latest developments in this emerging field. It is an invaluable reference for researchers working across biochemistry, enzymology, biotechnology, and related fields.

Considers the advantages and challenges of whole cell biocatalysis to enhance production processes in various industriesIncludes a range of techniques to investigate and facilitate whole cell biocatalysisCovers whole cell biocatalysis in non-conventional mediaStudies biocatalytic cascade reactions

RNases in Health and Diseases: From Housekeeping Enzymes to Moonlight Proteins provides coverage of the structural and functional properties of RNases, introducing new concepts such as their recently discovered multifaceted properties, their involvement in the generation of non-coding signaling RNAs, and their proposed uses as diagnostic and therapeutic tools. This book's sections cover the structure of RNases, present research methods and tools to study RNases, and delve into RNases and diseases. Users will find a wider perspective on the topics discussed that will serve researchers and students engaged in the study of RNases who are interested in acquiring a better understanding.

Covers basic theoretical information, methodological tools, and potential therapeutic applications in the RNase fieldIntroduces new concepts to the RNase fieldIncludes both the historical background in the field and the most recent advancesContains contributions from international experts in the field

Navigating Tuberculosis: Insights into Host-Pathogen Protein Interactions, the latest release in the Foundations and Frontiers in Enzymology series, explores the interactions between host proteins and the tuberculosis pathogen, Mycobacterium tuberculosis (M. tb), emphasizing their roles in virulence and pathogenesis. The book delves into mechanistic insights, focusing on their roles in modulating innate and adaptive immunity and understanding TB pathomechanisms by elucidating the interplay between host defenses and M. tb infection. It provides a foundation for developing targeted mitigation strategies and discusses key regulatory pathways and protein factors involved in host-pathogen interactions, informing the design and implementation of innovative approaches to combat TB.This comprehensive volume aims to advance knowledge and strategies to combat tuberculosis, aligning with the World Health Organization's goal of eradicating TB by 2035 by addressing the need for up-to-date information on host-pathogen interactions in tuberculosis and incorporating the latest research findings and advancements. It highlights emerging therapeutic targets among host and TB pathogen proteins and integrates multidisciplinary approaches from microbiology, immunology, and molecular biology, offering a holistic perspective on TB pathophysiology. Researchers will find detailed discussions on key host proteins involved in tuberculosis pathogenesis, the molecular tactics employed by M. tb to subvert host defenses, and host-mediated inhibition strategies targeting M. tb.Provides comprehensive insights into the complex interactions between host proteins and the tuberculosis pathogen, revealing essential aspects of tuberculosis pathophysiologyInvestigates the latest discoveries and breakthroughs in the field, presented by prominent authorities, to uncover innovative approaches for diagnosing and treating tuberculosis, including novel therapeutic targetsOffers a multidisciplinary perspective that combines insights from microbiology, immunology, biochemistry, and molecular biology, fostering a comprehensive understanding of host-pathogen interactions in tuberculosisHighlights cutting-edge research on key host and pathogen proteins involved in tuberculosis, offering detailed discussions on their roles in modulating innate and adaptive immunityExplores host-mediated inhibition strategies targeting Mycobacterium tuberculosis, paving the way for the development of novel therapeutic interventions to bolster host defenses against TB

Protein Dynamics: Experimental and Computational Approaches investigates protein biology from a biophysical standpoint. The book covers a wide range of topics, including functional protein dynamics, intrinsically disordered proteins, protein folding, misfolding and aggregation, and membrane protein-protein communication. Protein allosteric conformational changes caused by drug binding and disease-associated mutations are also featured, alongside protein dynamics in the context of computer-based drug design. Biophysical techniques for investigating protein structure-dynamics-function relationships across these topics are explored, considering the latest experimental and computational approaches in the field. This book is an ideal reference for researchers in working in biophysics, biochemistry, and related areas.Covers a broad range of biophysical techniques to study protein dynamicsInvestigates an array of different proteins, including receptors, transporters and enzymesConsiders future directions in the fieldFeatures explanation of key concepts and end-of-chapter questions to aid understanding

Enzyme Kinetics: Fundamentals, Applications, and Reactor Design is a comprehensive guide that provides readers with a solid grounding in the principles of enzyme kinetics and bioreactor design while also illuminating their practical applications. The book takes a step-by-step approach, starting from basic concepts and gradually moving to advanced topics, to ensure that readers develop a strong understanding and are well-equipped to apply this knowledge in real-world scenarios. “Enzyme Kinetics: Fundamentals, Applications, and Reactor Design” covers crucial areas including safety regulations and future trends, making it an all-in-one resource for anyone interested in these fields. It also provides foundational information, explains methods and computational approaches, and presents case studies and applications. This book is designed to serve as a valuable resource for a broad range of readers, including undergraduate and graduate students studying biochemistry, biotechnology, and chemical engineering, as well as professionals and researchers looking to deepen their knowledge and stay at the forefront of their field. Enzyme Kinetics: Fundamentals, Applications, and Reactor Design offers an integrative and up-to-date perspective on enzyme kinetics and bioreactor design, with an emphasis on real-world applications, cutting-edge developments, and safety regulations.Offers a complete and up-to-date overview of enzyme kinetics and bioreactor design, moving from fundamental concepts to advanced topics and covering crucial areas like safety regulations and future trends, making it an all-in-one resource for anyone interested in these fieldsUniquely bridges the gap between theory and practice, with real-world applications and case studies integrated throughout the content, enabling readers to directly apply what they learn to their work or researchPresents information in a clear and accessible manner, with concepts broken down step-by-step to ensure they are easy to grasp, making this book an invaluable tool for both beginners and experienced professionals alike

Carbonyl Reductase: Design, Screening, Modification and Industrial Applications promotes the practical applications of carbonyl reductase, introducing the most recent advances of carbonyl reductases, including the analyzed crystal structures and structural information, the catalytic mechanism, the high-throughput screening methods, the protein engineering and the expanded applications. It is a valuable resource for researchers and professionals interested in carbonyl reductase and chiral γ-amino alcohols production.

Super-Resolution Nanoscopy for Protein Imaging decodes the intricate world of protein visualization using cutting-edge nanoscopic techniques. This comprehensive guide is crafted to equip master students, PhD candidates, and early-stage researchers with essential knowledge and practical insights into advanced protein imaging technologies. The book covers foundational principles, innovative methodologies, and real-world applications, providing a complete resource for mastering the complexities of protein imaging. This book offers a unique compilation of diverse nanoscopic methods, overcoming the limitation of technique-specific texts currently available.By collecting scattered information into a single volume, it delivers an in-depth exploration of imaging techniques, from Atomic Force Microscopy (AFM) to Electron Microscopy (EM), and Single Molecule Fluorescence Imaging. Each section delves into instrumentation, sample preparation, data analysis, and the latest developments, making it an indispensable reference for understanding how to visualize proteins in their natural environments.Offers a comprehensive guide to various super-resolution nanoscopy techniques, providing in-depth knowledge on methodologies for imaging different types of proteinsExplains advanced instrumentation and sample preparation methods, ensuring readers can effectively utilize high-resolution imaging toolsDetails the strengths and limitations of various imaging techniques, helping researchers choose the most appropriate method for their specific needsShowcases real-world applications and case studies, demonstrating how these imaging techniques can be applied in biological research and future developmentsProvides insights into cutting-edge developments and future directions in protein imaging, empowering researchers to stay ahead in this rapidly evolving field